姜黄素对TNBS 诱导的大鼠结肠炎IL-36α和IL-36γ表达的影响

The effect of curcumin on the expression of IL-36αand IL-36γin TNBS induced colitis in rats

目的研究姜黄素对三硝基苯磺酸(TNBS)诱导大鼠结肠炎IL-36α和IL-36γ表达的影响。方法实验于2022年5月在广西医科大学医学实验动物中心进行。选取40只雄性SD大鼠随机分为正常对照组、模型组、姜黄素组、柳氮磺吡啶组,每组10只。模型组、姜黄素组、柳氮磺吡啶组大鼠分别以TNBS乙醇溶液灌肠制备大鼠结肠炎模型,正常对照组予等体积0.9%氯化钠溶液灌肠。姜黄素组、柳氮磺吡啶组自造模第2天起分别予姜黄素及柳氮磺吡啶溶液100 mg·kg^(-1)·d^(-1)灌胃,正常对照组、模型组每日予等体积的0.9%氯化钠溶液灌胃;给药7 d后处死,采集血清及结肠黏膜,比较各组大鼠体质量、疾病活动指数(DAI)、肠黏膜损伤指数(CMDI);ELISA法检测大鼠血清IL-36α、IL-36γ水平,免疫组化方法测定肠黏膜组织IL-36α和IL-36γ蛋白表达。结果与正常对照组比较,模型组DAI、CMDI评分均升高,差异有统计学意义(P均<0.01),血清IL-36α、IL-36γ水平均升高(P<0.01);与模型组比较,姜黄素组和柳氮磺吡啶组DAI、CMDI评分及血清IL-36α、IL-36γ水平均降低,且姜黄素组低于柳氮磺吡啶组,差异有统计学意义(F=3.531、3.842、24.503、36.435,P均<0.001)。与正常对照组比较,模型组结肠组织IL-36α、IL-36γ蛋白表达水平升高(P<0.05);与模型组比较,姜黄素组和柳氮磺吡啶组结肠黏膜IL-36α、IL-36γ蛋白表达水平降低,且姜黄素组低于柳氮磺吡啶组,差异有统计学意义(F=3.461、3.524,P均<0.001)。结论姜黄素能够明显抑制实验性大鼠结肠炎血清及结肠黏膜IL-36α、IL-36γ的表达。

Objective To investigate the effect of curcumin on the expression of IL-36αand IL-36γin colitis induced by trinitrobenzenesulfonic acid(TNBS)in rats.Methods The experiment was conducted in May 2022 at the Medical Experimental Animal Center of Guangxi Medical University.Forty male SD rats were randomly divided into a normal control group,a model group,a curcumin group,and a sulfasalazine group,with 10 rats in each group.The model group,curcumin group,and sulfasalazine group of rats were prepared with TNBS ethanol solution enema to create rat colitis models,while the normal control group was given an equal volume of 0.9%sodium chloride solution enema.The curcumin group and sulfasalazine group were orally administered with curcumin and sulfasalazine solutions of 100 mg·kg^(-1)·d^(-1) from the second day of modeling.The normal control group and model group were orally administered with an equal volume of 0.9%sodium chloride solution daily;After 7 days of administration,the rats were euthanized and serum and colon mucosa were collected.The body weight,disease activity index(DAI),and intestinal mucosal injury index(CMDI)of each group of rats were compared;ELISA method was used to detect the serum levels of IL-36αand IL-36γin rats,and immunohistochemical method was used to measure the protein expression of IL-36αand IL-36γin intestinal mucosal tissue.Results Compared with the normal control group,the DAI and CMDI scores of the model group increased significantly(P<0.01);The levels of serum IL-36αand IL-37 both increased(P<0.01);Compared with the model group,the DAI,CMDI scores,and serum IL-36αand IL-36γlevels in both the curcumin group and the sulfasalazine group decreased,and the curcumin group was lower than the sulfasalazine group,with statistically significant differences(F=3.531,3.842,24.503,36.435,P<0.001);Compared with the normal control group,the protein expression levels of IL-36 and IL-37 in the model group increased(P<0.05);Compared with the model group,the protein expression levels of IL-36αand IL-36γin the colon mucosa of the curcumin group and the sulfasalazine group decreased,and the curcumin group was lower than the sulfasalazine group,with statistical significance(F=3.461,3.524,P<0.001).Conclusion Curcumin can significantly inhibit the expression of IL-36αand IL-36γin serum and colon mucosa of experimental colitis rats.