盆腔炎患者感染高危型HPV的调查

Investigation of High-Risk HPV Infection of Patients with Pelvic Inflammatory Disease

目的了解盆腔炎患者感染高危型HPV的状况。方法采用美国ABI生物技术公司7500型全自动基因扩增仪检测人乳头瘤病毒DNA;采用DA8000型全自动核酸分子杂交仪进行人乳头瘤病毒DNA分型检测;所用试剂均由中山大学达安基因股份有限公司生产。结果资料显示300例盆腔炎患者中有96例人乳头瘤病毒阳性,阳性率为32.0%;HPV单一型感染率82.2%;多重感染率20.8%;高危型人乳头瘤病毒亚型中,感染率最高的是HPV-16型26人,其次为HPV-52型24人、HPV-33型10人;HPV-16型、HPV-52型、HPV-33型分别占人乳头瘤病毒阳性总阳性率的27.1%、25.0%、10.4%。结论盆腔炎患者中HPV感染远远高于我国妇女平均20%的感染率。高危型乳头瘤病毒亚型中,HPV-16型、HPV-52型、HPV-33型是我区关注的重点型别。

Objective To understand status of high-risk HPV infection of patients with pelvic inflammatory disease.Methods The paper detected human papillomavirus DNA with ABI Biotechnology 7500 fully automated gene amplification instrument from the US,detected human papillomavirus DNA typing with DA8000 fully automated nucleic acid molecular hybridization instrument;reagents were produced by Sun Yat-sen University Daan Gene Co.,Ltd.Results Data showed,96 out of 300 patients with pelvic inflammatory disease were positive for human papillomavirus,with a positive rate of 32.0%.Single type HPV infection rate was 82.2%;multiple infection rate was 20.8%.Among high-risk subtypes of human papillomavirus,26 individuals with HPV-16 had the highest infection rate,followed by 24 individuals with HPV-52,and 10 individuals with HPV-33.HPV-16,HPV-52,and HPV-33 accounted for 27.1%,25.0%,and 10.4%of total positive rates of human papillomavirus respectively.Conclusion HPV infection of patients with pelvic inflammatory disease is much higher than average infection rate of 20%among women in China.Among high-risk subtypes of papillomavirus,HPV-16,HPV-52,and HPV-33 are key types of concern in our region.