黄脊竹蝗胚胎发育与卵滞育发生阶段

Embryonic Development and Egg Diapause Stage of Bamboo Locust Ceracris kiangsu Tsai

[目的]探明黄脊竹蝗的胚胎发育特征、历期和卵滞育的发生阶段。[方法]采用体式显微镜观察黄脊竹蝗不同发育阶段的胚胎形态和发育历期,并对不同发育阶段蝗卵的含水量和胚胎长度、触角长度、头幅大小进行测定。[结果]在25℃恒温条件下,黄脊竹蝗的胚胎发育分为10个阶段,产卵后第2—5 d为第I阶段,第6—9 d为第Ⅱ阶段,第10—16 d为第Ⅲ阶段,第17—24 d为第IV阶段,第25—32 d为第V阶段,第33—75 d为第VI阶段,第76—85 d为第VII阶段,第86—92 d为第Ⅷ阶段,第93—100 d为第Ⅸ阶段,第101—109 d为第X阶段。胚胎发育可分为4个发育时期:第Ⅰ阶段和第Ⅱ阶段为胚胎起始发育期,第Ⅲ阶段和第Ⅳ阶段为胚胎的快速发育期,第Ⅴ—Ⅶ阶段为缓慢发育期,第Ⅷ—Ⅹ阶段为恢复发育期。产卵后第45 d之后卵粒进入滞育发育,卵滞育发生在第VI阶段。[结论]本研究明确了黄脊竹蝗胚胎发育历期及卵滞育发生的胚胎发育阶段,为明晰黄脊竹蝗卵滞育机制提供了基础信息。

[Objective]To explore the characteristics and duration of the embryonic development of Ceracris kiangsu Tsai and the occurrence stage of its egg diapause.[Method]The embryo morphology and development period of C.kiangsu at different developmental stages were observed by stereomicroscope,and the water content of eggs,the full length,antennal length and head size of the embryo of C.kiangsu at different developmental stages of the eggs were measured.[Results]Under constant temperature of 25℃,the embryonic development of C.kiangsu could be divided into 10 stages:stage Ⅰ(2-5 d),stage Ⅱ(6-9 d),stage Ⅲ(10-16 d),stage Ⅳ(17-24 d),stage Ⅴ(25-32 d),stage Ⅵ(33-75 d),stage Ⅶ(76-85 d),stage Ⅷ(86-92 d),stage Ⅸ(93-100 d)and stage Ⅹ(101-109 d).Embryonic development could be divided into four developmental stages:stage Ⅰ and stage Ⅱ were the initial development stage of embryos,stage Ⅲ and stage Ⅳ were the rapid development stage of embryos,stage Ⅴ-Ⅶ was the slow development stage,and stage Ⅷ-Ⅹ was the recovery development stage.After fourty-five days of oviposition,eggs entered the diapause development,and egg diapause occurred at stage VI.[Conclusion]This study clarifies the embryonic development period and the embryonic development stage of egg diapause in C.kiangsu,and provides data support for clarifying the mechanism of the egg diapause of C.kiangsu.