水稻愈伤组织诱导及不定芽分化培养体系建立

Induction of rice callus and establishment of adventitious bud differentiation culture system

目的:筛选高效诱导水稻愈伤组织及不定芽分化培养体系的培养基,为水稻基因工程育种奠定基础。方法:以优质水稻品种(润珠香占)成熟胚为外植体,研究成熟胚愈伤组织出愈情况、不同培养基以及植物生长物质浓度配比对愈伤组织诱导及不定芽分化的影响。结果:水稻愈伤诱导培养基出愈率大小为N6>B_(5)>MS>WPM,N_(6)与B_(5)、MS、WPM差异显著;2,4-D浓度愈伤组织诱导率大小为B_(4)>B_(3)>B_(5)>B_(6)>B_(2)>B_(1),B_(4)与各处理组之间差异显著;植物生长物质浓度配比对愈伤组织不定芽分化率大小为T_(5)>T_(7)>T_(6)>T_(1)>T_(8)>T_(3)>T_(2)>T_(4),T_(5)与各处理组之间差异显著。结论:水稻成熟胚愈伤诱导的最佳培养基及2,4-D质量浓度分别为N6、2.0 mg/L;不定芽分化最佳植物生长物质6-BA、KT、NAA质量浓度比为1∶1∶1,不定芽分化率最高。

Objective:Screening efficient medium for inducing rice callus and adventitious bud differentiation culture system,laying the foundation for rice genetic engineering breeding.Methods:Using mature embryos of high-quality rice variety(Runzhuxiangzhan)as explants,studied the callus induction of mature embryos,the effects of different media and plant growth substance concentrations on callus induction and adventitious bud differentiation.Results:The callus induction rate of rice medium was N_(6)>B_(5)>MS>WPM,N_(6) was significantly different from B_(5)、MS、WPM.The callus induction rate of 2,4-D concentration was B_(4)>B_(3)>B_(5)>B_(5)>B_(2)>B 1,significant difference between B_(4) and each treatment group.The ratio of plant growth substance concentration to Adventist bud differentiation of callus was T_(5)>T_(7)>T_(6)>T_(1)>T_(8)>T_(3)>T_(2)>T_(4),significant difference between T_(5) and each treatment group.Conclusion:The optimal culture medium and 2,4-D concentration for inducing rice mature embryo callus are N_(6),2.0 mg/L.The optimal concentration of plant growth substances for adventitious bud differentiation is 6-BA,KT,and NAA at a ratio of 1∶1∶1,with the highest rate of adventitious bud differentiation.