鲁氏接合酵母对高盐和高温胁迫响应的差异性与共性分析

Differences and Commonalities in Responses of Zygosaccharomyces rouxii to High Salt and High Temperature Stress

针对高温(40℃)和高盐(18%NaCl)逆境设计了最低营养需求全合成培养基,分析了鲁氏接合酵母(Zygosaccharomyces rouxii)在长期逆境下生长的营养需求差异,重点解析了酵母细胞从生长适应期到对数生长初期阶段有机酸、氨基酸和糖类物质的代谢及基因表达差异。研究结果显示,遭遇高盐压力的鲁氏接合酵母细胞更需要外源氨基酸,而补充维生素和氨基酸有助于缓解酵母细胞的高温压力。鲁氏接合酵母针对高盐和高温逆境采用了差异明显的有机酸、氨基酸和糖代谢策略。MSN4(逆境转录子基因)和HOG1(高渗调控蛋白基因)响应高盐,而HSF1(热激调控蛋白基因)和SOD1(超氧化物歧化酶基因)对高温响应。本研究加深了对耐盐鲁氏接合酵母耐温机制的理解,有助于双抗新能力酿造酵母菌株的研制。

Complete synthetic minimal media for Zygosaccharomyces rouxii growth were designed for high temperature(40℃,HTS)and high salt stress(18%NaCl,HSS)in this study,and the difference in the nutritional requirements of Z.rouxii cells under long-term adverse environmental conditions was analyzed.The differences in the metabolism and gene expression of organic acids,amino acids and sugars during the period from the growth adaptation stage to the early logarithmic stage were highlighted between HSS and HTS conditions.The results showed that Z.rouxii cells exposed to HSS needed more exogenous amino acids,vitamin and amino acid supplementation alleviated HTS-induced damage in yeast cells.The adversity transcription gene MSN4 and the hypertonic regulatory protein gene HOG1 responded to high salt,while the heat shock regulatory protein gene HSF1 and the superoxide dismutase gene SOD1 responded to high temperature.In summary,different strategies for organic acid,amino acid and sugar metabolism were adopted by Z.rouxii in response to HSS and HTS.This study deepens the understanding of the mechanism of temperature tolerance in salt-tolerant Z.rouxii,which will contribute to the development of new brewing yeast cells with tolerance to both high salt and temperature.