基于UPLC-Q-Orbitrap MS技术的沙棘黄酮类成分分析

Identification and Characterization of Flavonoid Compounds from Hippophae rhamnoides L.by UPLC-Q-Orbitrap MS

利用UPLC-Q-Orbitrap MS技术对沙棘(Hippophae rhamnoides L.)果实中黄酮类物质进行系统剖析,并对不同产地沙棘果实中黄酮类化合物进行比较分析,为中药沙棘的化学物质基础和资源利用研究奠定基础。根据保留时间比对,精确分子量匹配和MS/MS碎片信息分析,共鉴定出58个化合物,包括34个黄酮醇、4个黄酮、14个花色素、1个二氢黄烷酮、2个二氢黄烷醇、2个黄烷醇和1个二氢查尔酮,其中14个花色素类化合物为首次在沙棘果实中鉴定。最终筛选得到38种差异积累的黄酮类化合物,其中大部分在青海和西藏产地的沙棘果中呈显著积累。研究丰富了沙棘果中黄酮类化合物化学物质基础研究,揭示了不同产地沙棘果中黄酮类化合物的组成及差异。

The flavonoids in the fruits of Hippophae rhamnoides L.were systematically analyzed by UPLC-Q-Orbitrap MS technique,and the flavonoids in the fruits of Hippophae rhamnoides L.from different producing areas were compared and analyzed,which laid a foundation for the research on the chemical substance basis and resource utilization of traditional Chinese medicine Hippophae rhamnoides L.Based on retention time comparing,precise molecular weight matching and MS/MS fragment information analysis,a total of 58 compounds were identified,including 34 flavonols,4 flavones,14 anthocyanins,1 dihydroflavanone,2 dihydroflavanols,2 flavanols and 1 dihydrochalcones.Among them,14 anthocyanins were identified for the first time in Hippophae rhamnoides L.fruits.A total of 38 differentially accumulated flavonoids were finally screened,most of which were significantly accumulated in Hippophae rhamnoides L.fruits from Qinghai and Tibet.This study enriched the basic research of flavonoids in Hippophae rhamnoides L.fruit,and revealed the composition and differences of flavonoids in Hippophae rhamnoides L.fruit from different regions.