基于NF-κB通路探讨苗药酢浆草调控人前列腺癌PC-3细胞生物学作用的机制研究

Biological mechanisms of Oxalis corniculata regulating human prostate cancer PC-3 cells:An investigation based on the NF-κB pathway

目的:本研究旨在探讨苗药酢浆草对人前列腺癌PC-3细胞的生物学作用机制。方法:通过体外实验,用苗药酢浆草对PC-3细胞进行干预,采用了MTT法检测细胞活力,细胞流式技术检测PC-3细胞凋亡情况,Transwell法检测迁移及侵袭状况以及蛋白质印记技术检测NF-κB通路中p65、p-p65、Ikba、p-Ikba蛋白的表达情况。结果:苗药酢浆草以抑制PC-3细胞的增殖、诱导的PC-3细胞的凋亡,与对照组比较差异有统计学意义(P<0.05),苗药酢浆草呈浓度依赖性抑制PC-3细胞的侵袭与迁移,差异较对照组有统计学意义(P<0.05),同时上调IκBα,下调p-p65及p-IκBα的表达水平,差异较照对照组有统计学意义(P<0.05)。结论:苗药酢浆草可抑制PC-3细胞的增殖、迁移和侵袭,诱导PC-3细胞凋亡,且这些作用可能与抑制p-p65、p-Ikba的表达并调节NF-κB信号通路活性有关。

Objective:To investigate the biological mechanisms underlying the effect of the Chinese herbal medicine Oxalis corniculata on human prostate cancer PC-3 cells.Methods:Through in vitro experiment,we treated human prostate cancer PC-3 cells with different concentrations of Oxalis corniculata,assessed the viability of the cells by MTT assay,examined their apoptosis by flow cytometry,evaluated their migration and invasiveness by Transwell assay,and determined the expressions of the proteins p65,p-p65,IκBαand p-IκBαin the NF-κB pathway using protein imprinting technology.Results:Compared with the blank control,Oxalis corniculata significantly inhibited the proliferation and induced the apoptosis of the PC-3 cells(P<0.05),suppressed their migration and invasiveness in a dose-dependent manner(P<0.05),and upregulated the expression of IκBαand downregulated those of p-p65 and p-IκBαin the NF-κB pathway(P<0.05).Conclusion:Oxalis corniculata can inhibit the proliferation,migration and invasiveness and induce the apoptosis of human prostate cancer PC cells,which may be attributed to its abilities of inhibiting the expressions of p-p65 and p-IκBαand regulating the activity of the NF-κB pathway.