儿童配方奶粉对小鼠骨骼发育影响的研究

Effects of children growth milk powder on skeletal development in mice

该研究比较2种奶粉对地塞米松诱导小鼠骨骼发育不良的调节作用。选取雌性ICR小鼠,随机分为对照组、地塞米松组、地塞米松+奶粉1组、地塞米松+奶粉2组,分别给予相应饲料饲喂8周,采用肌肉注射1 mg/kg地塞米松建立骨骼发育不良模型,每周3次,实验结束时检测基础指标、血清生化指标和肠道菌群的变化。结果表明,与地塞米松组相比,2种奶粉添加后都能改善小鼠的骨微结构。血清生化分析表明,奶粉补充后血清骨转换标志物水平上升,血清促炎细胞因子水平下降,抗炎细胞因子水平增加,血清中钙含量增加。肠道菌群分析表明,奶粉补充后能显著改变肠道菌群的组成。在门水平上,奶粉1的添加增加了Verrucomicrobiota的相对丰度,奶粉2的添加增加了Firmicutes的相对丰度,降低了Bacteroidota的相对丰度。在属水平上,奶粉1的添加能上调Akkermansia和Alloprevotella的相对丰度,而奶粉2的添加能上调Lactobacillus和Lachnoclostridium的相对丰度。综上所述,2种奶粉都能促进骨骼发育,而调节机制存在差异,这可能与不同奶粉配方的独特组成有关。

This study aimed to compare the regulatory effects of two kinds of milk powder on skeletal dysplasia induced by dexamethasone in mice.Female ICR mice were randomly divided into a control group,dexamethasone group,dexamethasone+milk powder group 1,and dexamethasone+milk powder group 2,respectively.They were fed with corresponding diets for 8 weeks,and the skeletal dysplasia model was established by intramuscular injection of 1 mg/kg dexamethasone three times a week.At the end of the experiment,the changes in basic indexes,serum biochemical indexes,and intestinal flora were detected.Results showed that compared with the dexamethasone group,the two kinds of milk powder could improve the bone microstructure of mice.Serum biochemical analysis showed that milk powder supplementation increased serum bone turnover markers,decreased serum pro-inflammatory cytokines,and increased serum anti-inflammatory cytokines and serum calcium content.Intestinal microflora analysis showed that the composition of intestinal microflora could be changed after milk powder was supplemented.At the door level,the addition of milk powder 1 increased the relative abundance of Desulfobacterota,and the addition of milk powder 2 increased the relative abundance of Firmicutes but decreased the relative abundance of bacterioid OTA.At the genus level,the addition of milk powder 1 could increase the relative abundance of Akkermansia and Alloprevotella,while the addition of milk powder 2 could increase the relative abundance of Lactobacillus and Lachnoclostridium.To sum up,both kinds of milk powder can promote skeletal development,but there are differences in regulation mechanisms,which may be related to the unique composition of different milk powder formulas.