罗非鱼皮硫酸皮肤素与胶原蛋白同时提取工艺优化及初步鉴定

Optimization and preliminary identification of simultaneous extraction process of dermatan sulfate and collagen from tilapia skin

针对鱼皮单独提取硫酸皮肤素造成资源利用率不高的问题,该研究以罗非鱼皮为原料,采用低温酶提酸促溶法同时提取硫酸皮肤素与胶原蛋白,通过单因素试验及响应面优化提取工艺,并利用醋酸纤维素薄膜电泳、聚丙烯酰胺凝胶电泳、傅里叶变换红外光谱等对罗非鱼皮硫酸皮肤素与胶原蛋白进行初步鉴定。结果表明,最佳提取工艺为中性蛋白酶加酶量5930 U/g,提取时间25.8 h,提取温度28.5℃,料液比1∶40(g∶mL),在此条件下罗非鱼皮硫酸皮肤素得率为132.12 mg/100 g,胶原蛋白提取率为53.14%,二者分别具有典型的硫酸皮肤素及胶原蛋白特征。该研究为同时提取罗非鱼皮硫酸皮肤素与胶原蛋白提供了理论依据,为罗非鱼皮下脚料的高效利用提供了一种新途径。

In view of the low resource utilization caused by the extraction of dermatan sulfate from fish skin alone,this study used tilapia skin as the raw material,used low-temperature enzyme acid extraction and solubilization method to extract dermatan sulfate and collagen at the same time,optimized the extraction process through single-factor test and response surface,and preliminarily identified tilapia skin dermatan sulfate and collagen by cellulose acetate film electrophoresis,polyacrylamide gel electrophoresis(SDS-PAGE),and Fourier transform infrared spectroscopy(FTIR)spectra.Results showed that the optimum extraction conditions were as follows:neutral protease dosage 5930 U/g,25.8 h of extraction time,28.5℃of extraction temperature,and 1∶40(g∶mL)of solid-liquid ratio.Under these conditions,the yield of dermatan sulfate was 132.12 mg/100 g and the extraction rate of collagen was 53.14%.Both of them had typical characteristics of dermatan sulfate and collagen.This study provides a theoretical basis for the simultaneous extraction of dermatan sulfate and collagen in tilapia skin and provides a new way for the efficient utilization of tilapia skin scraps.