FBXO22表达对HPV阳性宫颈癌细胞生物学行为的影响

The Effect of FBXO22 expression on biological behavior of HPV positive cervical cancer cells

目的探索FBXO22表达对人乳头瘤病毒(HPV)阳性宫颈癌细胞生物学行为的影响。方法qRT-PCR和Western blotting检测FBXO22在正常宫颈细胞H8细胞和宫颈癌细胞系中的差异表达。实验组构建敲除和过表达FBXO22的Hela、CaSki细胞,对照组转入空载体。qRT-PCR和Western blotting检测转染后FBXO22表达;MTT、细胞克隆形成实验、流式细胞术、划痕实验和Transwell实验检测FBXO22表达对细胞增殖、细胞周期、迁移、侵袭能力的影响。结果与H8细胞比较,FBXO22在HPV阳性宫颈癌细胞中呈现过表达(P<0.05)。与对照组比较,Hela、CaSki细胞FBXO22 mRNA和蛋白、细胞增殖活性、细胞克隆形成率过表达组增加,敲低组减少(P<0.05);G2/M期细胞百分率、划痕宽度、穿膜细胞数过表达组减少,敲低组增加(P<0.05)。结论FBXO22在HPV阳性宫颈癌细胞中过表达,FBXO22表达促进宫颈癌细胞增殖和迁移,沉默FBXO22可能为宫颈癌治疗提供潜在靶点。

Aim To explore the effect of FBXO22 expression on the biological behavior of human papilloma virus(HPV)positive cervical cancer cells.Methods qRT-PCR and Western blotting were used to detect the differential expression of FBXO22 in H8 cells and cervical cancer cell lines.Hela and CaSki cells with FBXO22 knocked out and overexpressed were constructed in the experimental group,and the control group was transferred to empty vector.qRT-PCR and Western blotting were used to detect the expression of FBXO22 after transfection.MTT assay,cell clone formation test,flow cytometry,scratch test and Transwell test were used to detect the effects of FBXO22 expression on cell proliferation,cell cycle,migration and invasion.Results Compared with H8 cells,FBXO22 was overexpressed in HPV positive cervical cancer cells(P<0.05).Compared with the control group,the mRNA and protein of FBXO22,cell proliferation activity and cell clone formation rate of Hela and CaSki cells increased in the over-expression group,but decreased in the knock-down group(P<0.05).In G2/M phase,the percentage of cells,the width of scratches and the number of transmembrane cells decreased in FBXO22 overexpression group,but increased in knock-down group(P<0.05).Conclusion FBXO22 is overexpressed in HPV positive cervical cancer cells.The expression of FBXO22 promotes the proliferation and migration of cervical cancer cells,and silencing FBXO22 may provide a potential target for cervical cancer treatment.