薜荔叶化学成分及其活性研究

Studies on Chemical Constituents and Activities from Leaves of Ficus pumila

采用硅胶柱层析、ODS反相柱层析、Sephadex LH-20凝胶柱层析、半制备高效液相HPLC等色谱分离方法对薜荔(Ficus pumila)叶80%乙醇提取物进行分离纯化,得到7个化合物。根据化合物的理化性质和核磁共振波谱数据比对文献,分别鉴定为:3-hydroxy-damascone (1),月桂酸乙酯(2),马桑酸(3),13-hydroxy-(9Z,11E,15E)-octadecatrienoic acid (4),乙基-β-D-吡喃葡萄糖苷(5),苄醇-β-D-葡萄糖苷(6)和2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-propane-1,3-diol (7)。其中化合物7为首次从该属植物中分离得到,化合物1为首次从该植物中分离得到。抗肿瘤活性测试结果表明,化合物1对A549细胞显示出一定的抑制活性,IC50值为32.21μg/mL。抗菌活性测试结果表明,所有化合物对测试的4株人体致病菌和2株弧菌均无明显抗菌活性。

Seven compounds have been isolated from 80%ethanol extract of F.Pumila leaves by silica gel,ODS column chromatographies,Sephadex LH-20,and semi-preparative HPLC.Their structures were determined by physicochemical properties and spectral data analyses,and compared with literature data.The compounds were identified as 3-hydroxydamascone(1),ethyl laurate(2),coriolic acid(3),13-hydroxy-(9Z,11E,15E)-octadecatrienoic acid(4),ethyl-β-D-gluco⁃pyranoside(5),benzyl-β-D-glucopyranoside(6)and 2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-propane-1,3-diol(7).Compound 7 was isolated from the genus of Ficus for the first time,and coumpound 1 was isolated from F.Pumila for the first time.Compound 1 displayed the cytotoxicity against A549 cells with IC50 value of 32.21µg/mL.All compounds exhibited no antibacterial activity against the four human pathogenic bacterium and two vibrios.