北柴胡根部的化学成分研究

Chemical constituents from roots of Bupleurum chinense

目的对北柴胡Bupleurum chinense根中的化学成分进行研究。方法采用溶剂提取法、硅胶柱色谱及Sephadex LH-20凝胶柱色谱等方法进行分离纯化,运用现代波谱技术对化合物进行结构鉴定;采用MTT法对分离得到的单体成分进行活性筛选,初步评价了其对人肝癌HepG2细胞的增殖抑制作用。结果从北柴胡根部共分离得到19个化合物,分别鉴定为蛇床子素(1)、欧前胡素(2)、异补骨脂素(3)、对甲氧基桂皮酸乙酯(4)、反式-对羟基桂皮酸(5)、反式-3′,4′-亚甲二氧基桂皮酸(6)、反式-阿魏酸(7)、伞形花内酯(8)、七叶内酯(9)、迷迭香酸(10)、紫花前胡苷(11)、紫丁香苷(12)、5,7,3′,4′-四甲氧基黄酮(13)、5,7,4′-三羟基-6-异戊烯基异黄酮(14)、芹菜素(15)、木犀草素(16)、槲皮素(17)、牡荆素(18)和金丝桃苷(19)。结论化合物3~6、8、10~14、18和19首次从该植物中分离得到。化合物2、8~11、16和18对HepG2细胞具有明显的细胞毒活性,其半数抑制浓度(median inhibition concentration,IC50)值分别为38.07、6.91、29.41、22.86、48.99、15.91和30.82μmol/L。

Objective To study the chemical constituents from the roots of Bupleurum chinense.Methods The compounds were isolated by comprehensive column chromatography,and the structures were elucidated by spectral methods.The inhibitory proliferation effect of compounds on HepG2 were evaluated by MTT assay.Results Nineteen compounds were isolated from the roots of B.chinense.Their structures were elucidated as osthole(1),imperatorin(2),isopsoralen(3),4-methoxycinnamic acid ethyl ester(4),trans-4-hydroxycinnamic acid(5),trans-3′,4′-methylenedioxy cinnamic acid(6),trans-ferulic acid(7),umbelliferone(8),esculetin(9),labiatenic acid(10),marmesinin(11),syringoside(12),5,7,3′,4′-tetramethoxyflavone(13),5,7,4′-trihydroxy-6-isopentenyl isoflavone(14),apigenin(15),luteolin(16),quercetin(17),vitexin(18),hyperin(19).Conclusion Compounds 3—6,8,10—14 and 18—19 were isolated from this plant for the first time.Compounds 2,8—11,16 and 18 showed significant cytotoxicity against HepG2,with the IC50 values of 38.07,6.91,29.41,22.86,48.99,15.91 and 30.82μmol/L,respectively.