一测多评法测定大花龙胆中环烯醚萜类和黄酮类成分的含量

Determination of iridoids and flavonoids in Gentiana szechenyii by quantitative analysis of multi-components with single marker

目的拟用一测多评法同时测定大花龙胆Gentiana szechenyii中的环烯醚萜类成分和黄酮类成分的含量,并验证该方法在大花龙胆质量评价应用中的可行性和适用性。方法采用HPLC,以大花龙胆药材中2个典型成分大花龙胆苷A、异荭草苷为内参物,测定其与马钱苷酸、獐牙菜苷、乌奴龙胆苷D、平龙胆酯苷、异金雀花素-2″-O-吡喃葡萄糖苷、异金雀花素的相对校正因子,并用该相对校正因子计算其他几种成分的含量,实现一测多评;采用外标法测定药材中这8种成分的含量,并比较两者差异,以验证一测多评法的准确性和可行性;同时对不同产地药材质量进行评价。结果以大花龙胆苷A为内参物,马钱苷酸、獐芽菜苷、乌奴龙胆苷D、平龙胆酯苷的相对校正因子分别为1.901、0.994、0.974、1.398;以异荭草苷为内参物,异金雀花素-2″-O-吡喃葡萄糖苷、异金雀花素的相对校正因子分别为1.511、0.969。各相对校正因子重复性良好。一测多评法与外标法对24批大花龙胆中8个成分含量的测定结果无显著差异。结论所建立的一测多评法结果准确、可靠、重复性好,可为大花龙胆多指标质量评价提供参考。

Objective To determine the content of iridoids and flavonoids in Gentiana szechenyii simultaneously by quantitative analysis of multicomponents by single marker(QAMS),and verify the feasibility and applicability of the method in the application of its quality evaluation.Methods Using HPLC with two typical elements szechenyin A and isoorientin as the internal reference,the relative correction factors(RCFs)of the loganic acid,sweroside,gentiournoside D,depressine,isoscoparin-2″-β-D-glucopyranoside and isoscoparin were determined.Then,the above relative correction factors were used to calculate the content of several other components,so as to achieve QAMS.The external standard method(ESM)was used to determine the contents of these eight components in the medicinal materials.And the differences of measured results of the two methods were compared to verify the accuracy and feasibility of QAMS.The quality of herbs from different origins was also evaluated.Results The RCFs of the loganic acid,sweroside,gentiournoside D and depressine were 1.901,0.994,0.974 and 1.398,respectively,with szechenyin A as the internal reference.The RCFs of isoscoparin-2″-β-D-glucopyranoside and isoscoparin were 1.511 and 0.969,respectively,using isoorientin as the internal reference.The results showed that the RCFs repeatability was good.There were no significant differences in the determination results of the contents of 24 batches of G.szechenyii obtained by QAMS and ESM.Conclusion The results of the QAMS established in this study are accurate,reliable and reproducible,and can provide reference for the quality evaluation of multiple indicators of G.grandiflora.