根管生物膜体外模型研究进展

Research Progress on Root Canal Biofilm Models In Vitro

细菌生物膜是导致牙髓根尖周病及根管治疗失败的主要因素。建立体外根管生物膜模型是根管感染控制研究中的重要环节,模型的选择、设计与构建是否科学严谨直接影响研究的结果与意义。本文就近年来体外研究中常用的根管生物膜模型进行综述,讨论模型构建的各项因素,如细菌组成、载体的选择和预处理、细菌接种和培养条件等,以期为根管感染控制研究中选择和设计更科学、合理的模型,研发更能模拟体内情况、稳定可重复的标准模型提供参考。

Bacterial biofilm is the main cause of pulpal/periapical diseases and endodontic treatment failures.The establishment of in vitro root canal biofilm models is an important part of root canal infection control studies.Whether the selection,design,and construction of the models are scientific and rigorous directly affects the results and significance of the studies.This paper reviews the endodontic biofilm models commonly used in latest in vitro studies,and discusses the various constitutive factors,such as the microbial composition,the selection and pretreatment of substrates,and the inoculation and growth conditions,hoping to provide a reference for the selection and design of more scientific and sound models and the development of more stable and reproducible standardized models that can better mimic the in vivo conditions for future research.