癌组织中GPC-3、AUF1表达水平对食管癌患者病理分期及预后评估的意义

Significance of the Expression Levels of GPC-3 and AUF1 in Cancer Tissue in the Evaluation of Pathological Stage and Prognosis of Patients with Esophageal Cancer

目的探讨癌组织中磷脂酰肌醇蛋白聚糖-3(GPC-3)、AU碱基富集元件RNA结合因子1(AUF1)表达水平对食管癌患者病理分期及预后评估的意义。方法选取2017年11月—2019年11月收治的118例食管癌作为研究对象,收集癌组织(食管癌组)及对应癌旁组织(癌旁组)。采用免疫组化法检测癌组织及癌旁组织中GPC-3、AUF1表达水平,分析GPC-3、AUF1表达与食管癌患者临床病理特征的关系;采用多因素Cox回归分析探讨影响食管癌患者预后的危险因素。结果食管癌组GPC-3、AUF1阳性表达率(76.27%、74.58%)均高于癌旁组(38.98%、41.53%)(P<0.01)。低分化、TNM分期Ⅲ~Ⅳ期、有淋巴结转移食管癌患者GPC-3、AUF1阳性表达率分别高于中/高分化、TNM分期为Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05,P<0.01)。GPC-3阳性表达食管癌患者的3年生存率为63.33%,低于GPC-3阴性表达患者89.29%(P<0.01);AUF1阳性表达食管癌患者的3年生存率为62.50%,低于AUF1阴性表达患者90.00%(P<0.01)。TNM分期Ⅲ~Ⅳ期、有淋巴结转移、GPC-3阳性表达、AUF1阳性表达是影响食管癌患者预后的独立危险因素(P<0.01)。结论GPC-3、AUF1在食管癌组织中均呈高表达,且与临床病理特征及预后有关,可作为食管癌预后评估的生物学标志物。

Objective To investigate the significance of the expression levels of Glypican-3(GPC-3)and AU base-enriching element RNA binding factor 1(AUF1)in the pathological stage and prognosis of patients with esophageal cancer.Methods A total of 118 patients with esophageal cancer treated from November 2017 to November 2019 were selected as the research subjects,and cancerous tissues(esophageal cancer group)and corresponding para-cancerous tissues(para-cancer group)were collected.Immunohistochemistry was used to detect the expression levels of GPC-3 and AUF1 in cancerous and para-cancerous tissues,and the relationship between the expressions of GPC-3 and AUF1 and the clinicopathological features of patients with esophageal cancer was analyzed.Multivariate Cox regression was used to explore the risk factors affecting the prognosis of patients with esophageal cancer.Results The positive expression rates of GPC-3 and AUF1 in esophageal cancer group(76.27%,74.58%)were higher than those in para-cancerous group(38.98%,41.53%)(P<0.01).The positive expression rates of GPC-3 and AUF1 in patients with low differentiation,TNM stage Ⅲ-Ⅳ and lymph node metastasis were higher than those in patients with moderate/high differentiation,TNM stage Ⅰ-Ⅱ and no lymph node metastasis,respectively(P<0.05,P<0.01).The 3-year survival rate of patients with GPC-3-positive expression was 63.33%,which was lower than 89.29% of patients with GPC-3-negative expression(P<0.01).The 3-year survival rate of patients with AUF1-positive expression was 62.50%,which was lower than 90.00% of patients with AUF1-negative expression(P<0.01).TNM stage Ⅲ-Ⅳ,lymph node metastasis,positive expression of GPC-3 and positive expression of AUF1 were independent risk factors for the prognosis of patients with esophageal cancer(P<0.01).Conclusion Both GPC-3 and AUF1 are highly expressed in esophageal cancer tissues,and are related to clinicopathological features and prognosis,which can be used as biological markers for prognosis evaluation of esophageal cancer.