过表达CASP1诱导人急性髓系白血病细胞THP-1的G_(0)/G_(1)细胞周期阻滞和NLRP3炎性小体介导的焦亡

Overexpression of Caspase1 Induces G 0/G 1 Cell Cycle Arrest and NLRP3 Inflammasome-Mediated Pyroptosis in Human Acute Myeloid Leukemia Cells THP-1

目的:探讨半胱天冬酶1(Caspase1,CASP1)对人急性髓系白血病(acute myeloid leukemia,AML)细胞THP-1的细胞周期和细胞焦亡的影响。方法:培养THP-1细胞,将细胞分为对照组(正常培养的THP-1细胞),pcDNA3.1-null组(过表达CASP1的阴性对照,用5.0μg/mL的pcDNA3.1-null质粒转染THP-1细胞24h)、pcDNA3.1-CASP1组(过表达CASP1,用5.0μg/mL的pcDNA3.1-CASP1质粒转染THP-1细胞24h)。用CCK-8法检测各组细胞的增殖活力。用流式细胞术检测各组细胞凋亡和周期的变化。用qRT-PCR法检测细胞中CASP1、白细胞介素(interleukin,IL)-1β,IL-18的mRNA表达水平。用Western blot法检测细胞增殖相关蛋白Ki67、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA),细胞周期蛋白D1(cyclin D1)、NOD样受体热蛋白结构域相关蛋白3(Nod-like receptor heat protein domain associated protein 3,NLRP3)、凋亡相关斑点样蛋白((apoptosis-associated speck-like protein,ASC)、CASP1、切割半胱天冬酶1(cleaved-caspase 1,cleaved-CASP1)、IL-1β,IL-18、cleaved-Gasdermin D的相对表达水平。结果:与对照组比,pcDNA3.1-null组的细胞增殖活力、细胞凋亡、细胞周期变化均无统计学意义(P>0.05)。与对照组比,pcDNA3.1-CASP1组的细胞凋亡变化无统计学意义(P>0.05),细胞的增殖活力减少,G_(0)/G_(1)细胞周期被阻滞,Ki67、PCNA、cyclin D1的相对表达水平均减少(P<0.05),NLRP3、ASC、CASP1、cleaved-CASP1、IL-1β,IL-18、cleaved-Gasdermin D的相对表达水平均增加(P<0.05)。与pcDNA3.1-null组比,pcDNA3.1-CASP1组的细胞凋亡变化无统计学意义(P>0.05),细胞的增殖活力减少,G_(0)/G_(1)细胞周期被阻滞,Ki67、PCNA、cyclin D1的相对表达水平均减少(P<0.05),NLRP3、ASC、CASP1、cleaved-CASP1、IL-1β,IL-18、cleaved-Gasdermin D(30 kDA)的相对表达水平均增加(P<0.05)。结论:过表达CASP1诱导AML细胞THP-1的G_(0)/G_(1)细胞周期阻滞和NLRP3炎性小体介导的焦亡。

Objective:To investigate the effects of caspase1(CASP1)on the cell cycle and pyroptosis of human acute myeloid leukemia(AML)cells THP-1.Methods:THP-1 cells were cultured and divided into the control group(normal THP-1 cells),pcDNA3.1-null group(negative control for overexpression of CASP1,transfected with 5.0μg/mL pcDNA3.1-null plasmid into THP-1 cells for 24 h),and pcDNA3.1-CASP1 group(overexpression of CASP1,transfected with 5.0μg/ml pcDNA3.1-CASP1 plasmid into THP-1 cells for 24 h).Cell proliferation activity was detected by CCK-8 assay.Apoptosis and cell cycle were detected by flow cytometry.CASP1,interleukin(IL)-1β,and IL-18 mRNA expression levels were detected by qRT-PCR.Cell proliferation-related proteins Ki67,proliferating cell nuclear antigen(PCNA),cyclin D1,NOD-like receptor(NLR)-like receptor pyrin domain-containing protein 3(NLRP3),apoptosis-associated speck-like protein(ASC),CASP1,cleaved-caspase 1(cleaved-CASP1),IL-1β,IL-18,and cleaved-Gasdermin D were detected by Western blot.Results:Compared with the control group,there were no significant differences in cell proliferation activity,apoptosis,and cell cycle changes in the pcDNA3.1-null group(P>0.05).Compared with the control group,cell proliferation activity was reduced,G_(0)/G_(1) cell cycle was blocked,and the relative expression levels of Ki67,PCNA,and cyclin D1 were decreased(P<0.05)in the pcDNA3.1-CASP1 group.The relative expression levels of NLRP3,ASC,CASP1,cleaved-CASP1,IL-1β,IL-18,and cleaved-Gasdermin D were increased(P<0.05).Compared with the pcDNA3.1-null group,there were no significant differences in apoptosis in the pcDNA3.1-CASP1 group(P>0.05).However,cell proliferation activity was reduced,G_(0)/G_(1) cell cycle was blocked,and the relative expression levels of Ki67,PCNA,and cyclin D1 were decreased(P<0.05).The relative expression levels of NLRP3,ASC,CASP1,cleaved-CASP1,IL-1β,IL-18,and cleaved-Gasdermin D were increased(P<0.05).Conclusion:Overexpression of CASP1 induces G_(0)/G_(1) cell cycle arrest and NLRP3 inflammasome-mediated pyroptosis in AML cells THP-1.