土大黄对银屑病模型小鼠焦虑样行为学的影响

Effects of Rumicis Obtusifolii Radix on Anxiety-Like Behavior of Model Mice with Psoriasis

目的探讨土大黄对银屑病模型小鼠焦虑样行为学的影响。方法多次刺激复制焦虑小鼠模型,成功后用咪喹莫特乳膏涂抹小鼠背部脱毛区,以复制焦虑复合银屑病小鼠模型。将90只建模成功小鼠分为模型组(等体积生理盐水)、甲氨蝶呤组(1.3 mg/kg)、阿瑞匹坦组(5 mg/kg)及土大黄低、中、高剂量组(1,2,4 g/kg),各15只。各组小鼠灌胃相应药物或生理盐水,每天1次,连续15 d;另取15只小鼠设为正常对照组,无刺激,背部脱毛区涂抹凡士林,灌胃等体积生理盐水。取小鼠脑、心脏、肝脏、肾上腺、脾脏、胸腺,称定质量,并计算脏器系数;进行行为学实验;观察小鼠背部皮肤病理形态学,并进行评分。结果与模型组比较,阿瑞匹坦组、土大黄高剂量组小鼠肝脏系数及土大黄中剂量组小鼠肾脏系数均显著降低(P<0.05);土大黄各剂量组小鼠的中央区运动距离均显著延长,中央区/边缘区平均速率均显著提升,进入中央区次数均显著增加、潜伏期均显著延长(P<0.05);甲氨蝶呤组、阿瑞匹坦组及土大黄中、高剂量组小鼠新异臂、起始臂停留时间百分比均显著升高,其他臂停留时间百分比均显著降低(P<0.05);土大黄各剂量组小鼠真皮层及Baker评分,土大黄中、高剂量组小鼠表皮层评分,以及土大黄低、高剂量组小鼠角质层评分均显著降低(P<0.05)。与模型组比较,各给药组小鼠背部皮肤棘层增厚、角化不全及角化过度程度均减轻,未见明显表皮突延长及真皮乳头上移,且皮下炎性细胞浸润减少。结论土大黄不仅能缓解银屑病模型小鼠皮肤角质层、表皮层、真皮层损伤,同时能改善其焦虑、认知能力受损状态。

Objective To investigate the effect of Rumicis Obtusifolii Radix on the anxiety-like behavior of model mice with psoriasis.Methods Model mice with anxiety were replicated by multiple stimulation,and imiquimod cream preparation was applied to the depilated area on the back of mice to replicate the model mice with anxiety and psoriasis.A total of 90 model mice were divided into the model group(equal volume of physiological saline),the methotrexate group(1.3 mg/kg),the aprepitant group(5 mg/kg),the Rumicis Obtusifolii Radix low-,medium-and high-dose groups(1,2,4 g/kg),with 15 mice in each group.The mice in each group were given corresponding drugs or physiological saline by gavage once a day for 15 d.Another 15 mice without stimulation were selected as the normal control group,vaseline was applied to the depilated area on the back,and an equal volume of physiological saline was given by gavage.The brain,heart,liver,adrenal gland,spleen and thymus of mice were weighed,and the organ coefficients were calculated.The behavioral test was conducted.The skin pathological morphology on the back of mice was observed and scored.Results Compared with those in the model group,the liver coefficient in the aprepitant group and Rumicis Obtusifolii Radix high-dose group and the kidney coefficient in the Rumicis Obtusifolii Radix medium-dose group significantly decreased(P<0.05);the movement distance in the central area,average rate in the central and edge area,frequency of entry into the central area in the each dose group of Rumicis Obtusifolii Radix significantly increased,and the incubation period significantly prolonged(P<0.05).Compared with those in the model group,the percentage of residence time at the novel arm and start arm in the methotrexate group,aprepitant group,the Rumicis Obtusifolii Radix medium-and high-dose groups significantly increased,while the percentage of residence time at other arm significantly decreased(P<0.05);the stratum dermis score and Baker score in the each dose group of Rumicis Obtusifolii Radix,the stratum epidermis score in the Rumicis Obtusifolii Radix medium-and high-dose groups,and the stratum corneum score in the Rumicis Obtusifolii Radix low-and high-dose groups significantly decreased(P<0.05).Compared with those in the model group,the stratum spinosum thickening,parakeratosis and hyperkeratosis of the back skin in the methotrexate group,the aprepitant group and the each dose group of Rumicis Obtusifolii Radix improved,there was no obvious extension of epidermal protrusion and upward movement of dermal papillae,and the infiltration of subcutaneous inflammatory cells improved.Conclusion Rumicis Obtusifolii Radix can relieve the injuries of stratum corneum,stratum epidermis,stratum dermis of mice with psoriasis,and improve their anxiety and cognitive impairment.