补肾活血方对重度宫腔粘连术后患者子宫内膜干细胞标志物的影响

Effect of Bushen Huoxue Recipe(补肾活血方)on Endometrial Stem Cell Markers in Patients with Severe Intrauterine Adhesions

目的:探讨补肾活血方对重度宫腔粘连术后患者子宫内膜干细胞标志物表达的影响。方法:纳入重度宫腔粘连患者70例,采用随机数字表法分为对照组和治疗组,每组35例。对照组患者给予补佳乐及地屈孕酮片治疗,治疗组患者在此基础上加用补肾活血方,两组疗程均为3个月经周期。术后常规复查宫腔镜,比较干预前后两组患者子宫内膜组织中干细胞标志物的表达。结果:两组患者治疗后美国生殖协会(AFS)评分均显著降低,且治疗组患者治疗后AFS评分低于对照组(P<0.05);两组患者治疗后β-catenin蛋白、CD34蛋白、ABG2蛋白、Oct-4基因均表达均高于治疗前(P<0.05),且治疗组患者治疗后β-catenin蛋白、CD34蛋白、ABG2蛋白、Oct-4基因均表达高于对照组(P<0.01)。结论:补肾活血方可改善宫腔粘连分离术后宫腔粘连的预后,其治疗效果优于单独使用雌孕激素;补肾活血方可以增加β-catenin蛋白、CD34蛋白、ABG2蛋白、Oct-4基因的表达;补肾活血方可能通过调控Wnt/β-catenin信号通路影响子宫内膜干细胞的表达。

Objective:To investigate the effect of Bushen Huoxue Recipe on the expression of endometrial stem cell markers in patients with severe intrauterine adhesions.Methods:70 patients with severe intrauterine adhesions were randomly divided into two groups:the control group and the treatment group,35 cases in each.The control group was treated with Estradiol valerate and didrogestrel,while the treatment group received oral treatment with Bushen Huoxue Recipe.Both groups were treated for 3 menstrual cycles.Hysteroscopy was performed routinely after surgery,and the expression of stem cell markers in endometrial tissue between the two groups before and after intervention with traditional Chinese medicine were compared.Results:After treatment,the American Reproductive Society(AFS)scores of both groups significantly decreased,and the treatment group was significantly lower than the control group(P<0.05).After treatment,the expression ofβ-catenin protein,CD34 protein,ABG2 protein,and Oct-4 gene in both groups of patients was higher than before treatment(P<0.05),and the expression of these indicators in the treatment group was higher than that in the control group after treatment(P<0.01).Conclusion:Bushen Huoxue Recipe can improve the prognosis of postoperative uterine adhesions,and its therapeutic effect is superior to the use of estrogen and progesterone alone.Bushen Huoxue Recipe can increase the expression ofβ-catenin protein,CD34 protein,ABG2 protein,and Oct-4 gene,it may affect the expression of endometrial stem cells by regulating the Wnt/β-catenin signaling pathway.