猴头菌培养液乙酸乙酯提取物的化学成分研究

Chemical constituents of ethyl acetate extracts of the culture solution of Hericium erinaceus

目的研究猴头菌培养液乙酸乙酯提取物的化学成分。方法通过凝胶柱色谱、硅胶柱色谱、半制备高效液相色谱等技术进行分离和纯化;根据其理化性质数理和波谱对得到的化合物进行结构鉴定。结果从猴头菌培养液乙酸乙酯提取物中分离并鉴定得到22个化合物,分别为3-甲氧基-4-羟基苯甲醛(1),吲哚甲酸甲酯(2),2-呋喃甲酸(3),苯甲酸(4),2(3H)-苯并恶唑酮(5),香草酸(6),没食子酸(7),polisin C(8),bis(2-ethylhexyl)benzene-1,2-dicarboylate(9),p-tyrosyl acetate(4-hydroxyphenethyl acetate)(10),1-(4-hydroxy-3,5-dimethoxyphenyl)ethanon(11),对羟基苯乙酮(12),1-苯基-1,2-丙二醇(13),6-羟基异苯并呋喃-1(3H)-酮(14),对羟基苯乙醛(15),1H-吲哚-3-吡咯甲醛(16),环(脯氨酸-缬氨酸)(17),环(脯氨酸-亮氨酸)(18),环(苯丙氨酸-缬氨酸)(19),环(异亮氨酸-苯丙氨酸)(20),苜蓿素(21),dihydro-4-hydroxy-2(3H)-furanone(22)。结论除化合物16和21外,其他化合物均为首次从猴头菌培养液中分离得到。

Objective To study the chemical constituents of ethyl acetate extracts of the culture solution of Hericium erinaceus.Methods Compounds were isolated and purified using Sephadex LH-20 column chromatography,silica gel column chromatography,semi-preparative HPLC and so on,and their structures were identified based on their physicochemical properties and spectral data.Results A total of 22 compounds were isolated and obtained from the ethyl acetate extracts of the culture solution of Hericium erinaceus,including 3-methoxy-4-hydroxybenzaldehyde(1),methyl indole-3-carboxylate(2),2-furancarboxylic acid(3),benzoic acid(4),2(3H)-benzoxazolone(5),vanillic acid(6),gallic acid(7),polisin C(8),bis(2-ethylhexyl)benzene-1,2-dicarb-oxylate(9),p-tyrosyl acetate(4-hydroxyphenethyl acetate)(10),1-(4-hydroxy-3,5-dimethoxyphenyl)ethanon(11),hydroxyacetophenone(12),1-phenyl-1,2-propanediol(13),6-hydroxy isobenzofuran-1(3H)-one(14),4-hydroxyphenylacetaldehyde(15),1H-indole-3-carbox-aldehyde(16),cyclo(Pro-Val)(17),cyclo(Pro-Leu)(18),cyclo(Phe-Val)(19),cyclo(Ile-Phe)(20),3',5'-dimethoxy-4',5,7-trihydroxyflavone(21),and dihydro-4-hydroxy-2(3H)-furanone(22).Conclusion With the exception of compounds 16 and 21,all other compounds were isolated from the culture solution of Hericium erinaceus for the first time.