多发性硬化症发作期外周血和脑脊液淋巴细胞亚群的分析

Analysis of Lymphocyte Subsets of Peripheral Blood and Cerebrospinal Fluid in Relapse Phase of Multiple Sclerosis

为分析多发性硬化症 (MS )患者发作期淋巴细胞亚群及给予甲基强的松龙 (MP )治疗后的变化 ,流式细胞仪测定 2 6例处于复发期MS患者外周血 (PB )和脑脊液 (CSF )及 8例MS患者予MP治疗后PB淋巴细胞CD3+ 、CD4 + 、CD8+ 、CD4 5RA+ 、CD4 + /CD4 5RA+ 、CD4 + /CD2 9+ 、CD19+ 、CD5 + /CD19+ 的百分率。结果发现MS患者PB中CD8+ 、CD4 5RA+ 和CD4 + /CD4 5RA+ 百分率降低 ,CD4 + /CD2 9+ 百分率和CD4 + /CD8+ 比值升高 ;CSF中CD3+ 、CD4 + 、CD4 + /CD2 9+ 百分率和CD4 + /CD8+ 比值高于PB ;淋巴细胞亚群与临床伤残程度和距此次发作的时间无关 ;MP治疗不影响PB淋巴细胞亚群变化。表明MS患者淋巴细胞通过血脑屏障有选择性 。

To analysis the changes of lymphocyte subsets in active phase and after methylprednisolone (MP) treatment in patients with multiple sclerosis (MS),the percentage of CD3 +,CD4 +,CD8 +,CD45RA +,CD4 +/CD45RA +,CD4 +/CD29 +,CD19 +,CD5 +/CD19 + lymphocytes were investigated in peripheral blood (PB) and cerebrospinal fluid (CSF) by flow cytometry from 26 patients with relapse phase MS and 22 age-,sex-match controls The results showed that the percentage of CD8 +,CD45RA + and CD4 +/CD45RA + of PB were significantly decreased in MS patients than in controls;the percentage of CD4 +/CD29 + and CD4 +/CD8 + ratio were higher compared with controls In MS patient s,a significant increase percentage of CD3 +,CD4 +,CD4 +/CD29 + and CD4 +/CD8 + ratio were found in CSF comparison with in PB There was no significant correlation between the phenotype of lymphocyte subsets and clinical expanded disability scale score (EDSS),time of onset of clinical relapse After MP treatment,lymphocyte subsets did not significantly changed Our observations support the notion that lymphocytes selectively pass the blood-brain barrier in MS patient,and the changes of lymphocyte subsets involve in pathogenesis of MS -