高效液相色谱法测定敌草快二溴盐中2,2′-联吡啶含量的研究-联吡啶含量的研究

Quantitative Measurement of 2,2′-Bipyridine in Diquat Dibromide by HPLC

建立了敌草快二溴盐中2,2′-联吡啶的液相色谱检测方法。以shim-pack-VP-ODS 250 mm×5.4 mm(I.D)×5μm色谱柱分离,经紫外可变波长检测器进行敌草快二溴盐中2,2′-联吡啶的测定。当敌草快二溴盐中2,2′-联吡啶的质量浓度为0.2~50 mg/L时,具有良好的线性。对4个不同添加水平下的添加回收率进行测定,结果表明,2,2′-联吡啶在敌草快二溴盐中添加回收率为86.8%~103.6%,RSD为2.7%~9.0%。该方法满足农药检测要求,适用于敌草快二溴盐中2,2′-联吡啶含量的检测。

A HPLC method for the quantitative measurement of 2,2′-bipyridine in diquat dibromide was established.2,2′-bipyridine in diquat dibromide was separated by Shim-Pack-VP-ODS 250 mm×5.4 mm(I.D.)×5μm column,and determined by ultraviolet variable wavelength detector.When the mass concentration of 2,2′-bipyridine in diquat dibromide is 0.2~50 mg/L,good linearity was presented.The recovery of 2,2′-bipyridine in diquat dibromide was determined at four different levels.The results showed that the recovery of 2,2′-bipyridine was 86.8%~103.6% with RSD of 2.7%~9.0%.The method meets the requirements of pesticide detection,and is suitable for the detection of 2,2′-bipyridine in diquat dibromide.