云南野生余甘子鞣花酸和漆黄素的减肥降脂作用

9078.2024.1.1193Anti-obesity and Lipid-lowering Effects of Ellagic Acid and Fisetin from Yunnan’s Wild Phyllanthus emblica

余甘子(Phyllanthus emblica,PE)是近年广受关注的药食两用水果,但关于其减肥降脂功效及具体作用成分鲜有报道。该研究提取了云南野生PE富含的黄酮多酚物质(Phyllanthus Emblica Extract,PEE),发现其在小鼠前脂肪细胞(3T3-L1)模型中,能够显著抑制3T3-L1细胞分化至成熟脂肪细胞,减少脂滴生成量,300μg/mL作用时,细胞总甘油三酯(Triglyceride,TG)、总胆固醇(Total Cholesterol,TC)相对含量分别降低至66.47%、40.87%,这表明,云南野生PE中的PEE具有减肥降脂潜力。通过HPLC分析测得,云南野生PE中含有3030.78μg/g鞣花酸,560.70μg/g漆黄素。将鞣花酸和漆黄素以5:1的比例还原搭配成混合物(Ellagic Acid and Fisetin,EF),研究其对高脂饮食(High Fat Diet,HFD)小鼠模型的影响。结果显示,EF能够抑制HFD小鼠的体质量和白色脂肪增长,高剂量干预时,血清TG、TC、LDL-C含量分别降低了45.48%、12.06%、37.54%,HDL-C水平提高了18.75%;HFD小鼠的肝脏指数、血清中ALT和AST分别降低了7.80%、21.59%、41.64%。EF具有减肥降脂功效,且对肝脏具有一定的修复作用,是云南野生PE减肥降脂作用的重要成分。

Phyllanthus emblica(PE)is an edible and medicinal fruit that has received much attention in recent years,but little has been reported about its anti-obesity and lipid-lowering effects as well as specific functional components.In this study,the flavonol-rich polyphenols from Yunnan wild PE were extracted(Phyllanthus emblica Extract,PEE).It was found that in the 3T3-L1 cell model,PEE could significantly inhibit the differentiation of 3T3-L1 cells into mature adipocytes and reduce the production of lipid droplets.When PEE at 300μg/mL was applied,the relative contents of total triglyceride(TG)and total cholesterol(TC)in cells were reduced to 66.47%and 40.87%,respectively,suggesting that PEE in Yunnan wild PE has the potential to promote weight loss and lipid reduction.HPLC analysis revealed that Yunnan wild PE contained ellagic acid(3030.78μg/g)and fisetin(560.70μg/g).Ellagic acid and fisetin were mixed at a 5:1 ratio to form a mixture(EF),and the effect of EF on a high-fat diet(HFD)mouse model was examined.The results showed that EF could inhibit the growth of body weight and white fat in HFD mice,and decreased the serum TG,TC and LDL-C contents by 45.48%,12.06%and 37.54%,respectively,while increasing the HDL-C level by 18.75%.The liver index,serum ALT and AST of the HFD mice decreased by 7.80%,21.59%and 41.64%,respectively.EF exhibited anti-obesity and lipid-lowering effects,and could exert certain repairing functions on the liver,thus,EF are important components accounting at least paritially for the anti-obesity and lipid-lowering effects of Yunnan wild PE.