基于生信分析纳米银对斑马鱼的毒性和机制

Toxicity effects of silver nanoparticles on zebrafish and the underlying mechanisms based on bioinformatics analysis

依托GEO数据库的GSE50718、GSE61186和GSE89653数据集,从中提取斑马鱼的转录组相关数据,通过生物信息学分析鉴定不同粒径和涂层的纳米银对斑马鱼进行暴露产生的显著差异基因,并比较相关差异基因的数量和上、下调关系.研究发现,斑马鱼的差异表达基因数量按从多到少的排序为:PVP-AgNPs-50nm> AgNO_(3)> PVP-AgNPs-150nm;Maltose-AgNPs> AgNPs.通过GO、KEGG和GSEA富集分析,本研究发现纳米银差异基因主要在免疫相关通路中有显著富集,而硝酸银则主要影响细胞周期及代谢途径.这一发现暗示纳米银与硝酸银在分子层面上的作用机制存在明显的差异.推测纳米银的毒性作用不仅源于释放的银离子,还与其“颗粒特异性”紧密相关.此外,纳米银的表面涂层亦对其毒性产生影响.这些发现为纳米银的安全有效应用提供了有益的参考和策略性建议.

By employing the datasets(GSE50718,GSE61186,and GSE89653)from the GEO database,the underlying transcriptomic data associated with zebrafish(Danio rerio)were rigorously extracted and analyzed.Through bioinformatics analysis,we identified the significantly differentially expressed genes(DEGs)in zebrafish when exposed to silver nanoparticles with varying sizes and coatings,and subsequently compared the quantity and expression trends of these DEGs.Consequently,the abundance of DEGs was in the following order:PVP-AgNPs-50nm>AgNO3>PVP-AgNPs-150nm;Maltose-AgNPs>AgNPs.By virtue of the enrichment analysis(GO,KEGG,and GSEA),it was observed that under the exposure of silver nanoparticles,the DEGs were predominantly enriched in the immune-related pathways.In sharp contrast,silver nitrate primarily affected zebrafish cell cycle and metabolic pathways.These findings provided compelling evidence that a distinct difference in molecular mechanism existed between silver nanoparticles and silver nitrate.Also,the aforementioned results led us to infer that the toxicity of silver nanoparticles not only results from the release of silver ions,but also from their inherent"particle-specificity".Moreover,the surface coating of silver nanoparticles played an important role in modulating their toxicity.These insights provide valuable guidance and strategic suggestions for the safe and effective application of silver nanoparticles in various fields.