秦艽中1个新的苯并吡喃糖苷类化合物

A new benzopyran glycoside from Gentiana macrophylla

采用大孔树脂、硅胶、RP-C_(18)柱色谱及HPLC等色谱分离技术从秦艽Gentiana macrophylla的70%乙醇提取物中分离得到13个化合物,分别鉴定为macrophylloside G (1)、macrophylloside D (2)、5-formyl-2,3-dihydroisocoumarin (3)、(+)-medicarpin (4)、(+)-syringaresinol (5)、liquiritigenin (6)、(3R)-sativanone (7)、(3R)-3′-O-methylviolanone (8)、4,2′,4′-trihydroxychalcone (9)、latifolin (10)、gentioxepine (11)、6α-hydroxycyclonerolidol (12)、ethyl linoleate (13)。其中化合物1为1个新的苯并吡喃糖苷类化合物,化合物4、6~10、12和13均为首次从秦艽和龙胆属中分离得到。化合物1和2在10μmol·L^(-1)浓度下对用D-GalN诱导的AML12细胞肝损伤模型具有显著的保护活性,其中化合物2比同浓度下的水飞蓟宾活性更加显著。

Thirteen compounds were isolated and identified from 70% ethanol extract of the roots of Gentiana macrophylla by multi-chromatographic methods,including microporous resin,silica gel,and C_(18) reversed-phase column chromatography,as well as HPLC as follows:macrophylloside G(1),macrophylloside D(2),5-formyl-2,3-dihydroisocoumarin(3),(+)-medicarpin(4),(+)-syringaresinol(5),liquiritigenin(6),(3R)-sativanone(7),(3R)-3′-O-methylviolanone(8),4,2′,4′-trihydroxychalcone(9),latifolin(10),gentioxepine(11),6α-hydroxycyclonerolidol(12),and ethyl linoleate(13).Compound 1 was a new benzopyran glycoside.Compounds 4,6-10,12,and 13 were isolated for the first time from Gentiana plants.Compounds 1 and 2 showed promising hepatoprotective activity against D-GalN-induced AML12 cell damage at the concentration of 10 μmol·L~(-1),and compound 2 exhibited more significant activity than silybin at the same concentration.