HOXB4基因表达水平在骨髓增生异常综合征中的临床意义

Clinical significance of HOXB4 gene expression levels in myelodysplastic syndromes

目的探讨HOXB4基因在骨髓增生异常综合征(myelodysplastic syndromes,MDS)患者中的表达情况及其在疾病进展中的临床意义。方法利用实时荧光定量PCR(RT-qPCR)法检测骨髓单个核细胞HOXB4基因的mRNA表达水平,对比49例MDS患者(MDS组)和35例非恶性血液系统疾病患者(C组)的HOXB4基因的表达差异,并探讨HOXB4的表达水平与MDS疾病特征及临床预后之间的关系。结果MDS组HOXB4基因的表达水平较C组高(P<0.05)。根据HOXB4的中位表达水平分为高表达组和低表达组,高表达组患者初诊时白细胞水平低于低表达组,而伴原始细胞增多的MDS亚型、不良预后分型及白血病转化的患者比例高于低表达组(P<0.05)。结论HOXB4基因的表达水平与MDS患者向急性白血病转化有一定关联性。

Objective To investigate the expression of HOXB4 gene in patients with myelodysplastic syn⁃dromes(MDS)and its clinical significance in disease progression.Methods mRNA expression of HOXB4 gene in bone marrow mononuclear cells was detected by real⁃time fluorescence quantitative PCR(RT⁃qPCR),and the difference in HOXB4 expression was compared between 49 patients with MDS(MDS group)and 35 patients without MDS(group C).The relationship of mRNA expression of HOXB4 with disease characteristics and clinical prognosis was explored in MDS patients.Results mRNA expression level of HOXB4 gene was higher in MDS group than that in group C(P<0.05).The patients were divided into a high⁃and a low⁃expression group according to the median expression level of HOXB4.Leukocyte count was lower in the high⁃expression group in the low⁃expression group at the time of initial diagnosis.The proportion of patients with subtypes of primitive cellular hyperplasia,poor prognostic staging and leukemic transformation was higher in the high⁃expression group than in the low⁃expression group.Conclusions mRNA expression level of HOXB4 gene has certain relation with AML transformation in MDS patients.