负载葡萄籽多酚海藻酸钠冻干凝胶的制备及其抗菌止血性能

Preparation of Sodium Alginate Freeze-Dried Gel Loaded with Grape Seed Polyphenols and Its Properties of Antimicrobial and Hemostatic

以海藻酸钠(SA)、葡萄籽提取物(GSE)、Fe3+为原料,经过两次冷冻干燥得到冻干凝胶SA/GSE/Fe3+(SGF(0.75)),并通过体外实验探究SGF(0.75)冻干凝胶的孔隙率、吸水率、光热特性、抗菌性能、生物相容性及止血性能。结果表明,SGF(0.75)的孔隙率约37%,吸水率约1500%。在近红外激光照射下,SGF(0.75)可以杀死近90%的金黄色葡萄球菌和91%的大肠杆菌。止血性能实验表明,与纱布相比,SGF(0.75)失血量和止血时间分别减少了31%和66%。SGF(0.75)冻干凝胶止血能力强,抗菌效果好,且生物相容性好,安全性高,能促进伤口组织的愈合。

A freeze-dried gel containing sodium alginate loaded grape seed extract was prepared and its antibacterial and hemostatic properties were investigated.The freeze-dried gel SA/GSE/Fe3+(SGF(0.75))was obtained with the mixture of grape seed extract(GSE),sodium alginate(SA)and Fe3+by being freeze-dried twice.Then the porosity,water absorption,photothermal properties,antibacterial properties,biocompatibility and hemostatic properties of SGF(0.75)freeze-dried gel were investigated through in vitro experiments.The prepared SGF(0.75)freeze-dried gel has a porosity of about 37%and a water absorption rate of about 1500%.Under near-infrared laser irradiation,SGF(0.75)freeze-dried gel can kill nearly 90%of Staphylococcus aureus and 91%of Escherichia coli.The hemostatic performance experiment shows that SGF(0.75)freeze-dried gel reduces blood loss and hemostatic time by 31%and 66%,respectively,in comparism with gauze.The prepared SGF(0.75)freeze-dried gel has strong hemostatic ability,good antibacterial effect,good biocompatibility,high safety,and can promote wound tissue healing.It has great application prospects in future clinical research.