胚自主发生基因BBM1在水稻无融合生殖中的应用研究

Application of Autonomous Embryonic Gene BBM1 in Apomixis of Rice

固定F1代杂种优势是杂交水稻育种的战略目标和发展方向,而利用无融合生殖是固定杂种优势最有可能的途径。本研究利用组成型启动子Ubiq驱动胚自主发生基因BBM1,通过流式细胞倍性检测及基因芯片分析,发现T1代株系中出现单倍体植株;随后将卵细胞特异启动子AtDD45驱动BBM1基因表达盒,构建到PAIR1、REC8和OSD1(MiMe)基因编辑载体,获得了克隆种子,其诱导率高达95.24%。本研究为水稻无融合生殖技术体系的应用提供了参考。

Fixation of the heterosis of F1 generation is the strategic goal and development direction of hybrid rice breeding,and apomixis is the most possible way to fix heterosis.In this study,the autonomous embryonic gene BBM1 was driven by constitutive promoter Ubiq.Through flow cytometry ploidy detection and gene chip analysis,haploid plants were found in T1 strains.Subsequently,the BBM1 gene expression box was driven by the egg-specific promoter AtDD45 and constructed into the gene editing vectors of PAIR1,REC8 and OSD1(MiMe).Cloned seeds were obtained,and the induction rate was as high as 95.24%.This study provides a reference for the application of apomixis technology system in rice.