高分辨质谱技术分析两地缢蛏的脂质差异性

High resolution mass spectrometry analysis of lipid differences in Sinonovacula constricta from two regions

目的基于高分辨质谱技术对浙江象山和福建云霄两大主产地缢蛏的脂质差异性进行比较。方法样品经培养后,去壳、去胃进行冷冻干燥,采用脂类提取法提取,以Hypersile Gold C18(100 mm×2.1 mm,1.9μm)色谱柱为分析柱,甲醇/水(A)、甲醇/异丙醇(B)为流动相进行梯度洗脱分离,采用四极杆静电场轨道阱高分辨质谱法对缢蛏脂质进行定性和半定量分析。结果两大主产地缢蛏均检测到脂质78种,潜在生物标记物由磷脂酰乙醇胺(phosphatidyl ethanolamine,PE)、磷脂酰肌醇(phosphatidylinositol,PI)、磷脂酰丝氨酸(phosphatidylserine,PS)、神经酰胺氨乙基亚磷酸(ceramide aminoethyl phosphite,CAEP)、甘油三酯(triglyceride,TAG)和甘油二酯(diglyceride,DAG)组成。浙江象山产地的缢蛏PE(17:1-22:6,15:1-22:6,16:1-22:6,17:1-18:4)、PI(20:3-20:5)和DAG(18:4-18:4)含量为福建云霄产地的2.55~9.11倍;福建云霄产地的缢蛏DAG(16:0-16:0)、CAEP(d18:2-n16:0)和PI(16:0-20:5)含量是浙江象山产地的2.79~2.90倍。结论浙江象山和福建云霄产地的缢蛏存在脂质差异性,为其产地鉴定提供重要依据。

Objective To compare the differences in lipids of Sinonovacula constricta from 2 main producing areas of Xiangshan in Zhejiang and Yunxiao in Fujian based on high resolution mass spectrometry technology.Methods After the sample was cultured,the shell and stomach were removed for freeze-drying,and the lipid extraction method was used for extraction.Hypersile Gold C 18(100 mm×2.1 mm,1.9μm)chromatographic column was regarded as the analysis column,and methanol/water(A)and methanol/isopropanol(B)was treated as the mobile phases for gradient elution separation.The lipids of Sinonovacula constricta were qualitatively and semi-quantitatively analyzed by quadrupole/electrostatic field orbitrap high resolution mass spectrometry.Results The 78 kinds of lipids were detected in the Sinonovacula constricta from 2 main producing areas,potential biomarkers were composed of phosphatidyl ethanolamine(PE),phosphatidylinositol(PI),phosphatidylserine(PS),ceramide aminoethyl phosphate(CAEP),triglycerides(TAG),and diglycerides(DAG).The content of PE(17:1-22:6,15:1-22:6,16:1-22:6,17:1-18:4),PI(20:3-20:5),and DAG(18:4-18:4)in the Sinonovacula constricta from Xiangshan area of Zhejiang was 2.55-9.11 times higher than that from the Yunxiao area of Fujian;while the content of DAG(16:0-16:0),CAEP(d18:2-n16:0),and PI(16:0-20:5)in the Sinonovacula constricta from Yunxiao area of Fujian were 2.79-2.90 times higher than those of Xiangshan area in Zhejiang.Conclusion There are lipid differences between Sinonovacula constricta from Xiangshan in Zhejiang and Yunxiao in Fujian,providing important evidence for their origin identification.