发酵粘液乳杆菌IOB802对小鼠骨骼生长的影响

Effects of Limosilactobacillus fermentum IOB802 on bone growth in mice

目的探究发酵粘液乳杆菌(Limosilactobacillus fermentum)IOB802对骨骼生长的影响。方法以IOB802的后生元粉、冻干粉、多糖为实验对象,将无特定病原体小鼠(specific pathogen free,SPF)级雄性小鼠分别灌胃相同剂量的不同样品,测定了小鼠的一般形态、股骨长度、骨重和骨密度,血清胰岛素生长因子(insulin-like growth factor-1,IGF-1)和血清骨钙素(osteocalcin,OCN)水平。结果不同处理组对小鼠体重无明显影响,3种样品均能够促进小鼠身长增长,均能够增加6.96%以上的股骨长度,6.77%以上的骨重,10.04%以上的骨密度,均能够提高血清中IGF-1和OCN水平。其中灌胃发酵粘液乳杆菌IOB802后生元粉表现较好。结论以发酵粘液乳杆菌IOB802发酵后生元粉,发酵粘液乳杆菌IOB802冻干粉,发酵粘液乳杆菌IOB802多糖均具有良好的增加股骨长度、骨重和骨密度、血清IGF-1和OCN水平的功能,本研究为深入探究发酵粘液乳杆菌IOB802提供了一定的理论基础。

Objective To investigate the effects of Limosilactobacillus fermentum IOB802 on bone growth.Methods The general morphology,femur length,bone weight and bone mineral density,serum insulin-like growth factor-1(IGF-1)and serum osteocalcin(OCN)levels were determined by gavage of different samples at the same dosage in male mice of specific pathogen free(SPF)class using the powdered,lyophilized and polysaccharides of the postbiotic IOB802 as the experimental subjects.Results The different treatment groups had no significant effect on the body weight of mice,and all 3 kinds of samples were able to promote the growth of mouse length,which could increase femur length by more than 6.96%,bone weight by more than 6.77%,bone density by more than 10.04%,and all of them were able to increase the levels of IGF-1 and OCN in serum.Among them,gavage fermented Limosilactobacillus fermentum IOB802 postbiotic powder performed better.Conclusion Limosilactobacillus fermentum IOB802 fermented postbiotic meta-powder,Limosilactobacillus fermentum IOB802 lyophilized powder,Limosilactobacillus fermentum IOB802 polysaccharide supernatant all had good functions in increasing femur length,bone weight and bone mineral density,and serum IGF-1 and OCN levels,and the present study provided some theoretical bases for the in-depth investigation of Limosilactobacillus fermentum IOB802.