淫羊藿治疗骨质疏松骨折的网络药理学分析

Network Pharmacology Analysis of Epimedium in the Treatment of Osteoporotic Fracture

目的利用网络药理学原理探讨淫羊藿治疗骨质疏松骨折的分子作用机制。方法利用中药系统药理学分析平台(TCMSP数据库)筛选淫羊藿的主要活性成分和作用靶点,应用GeneCards数据库、OMIM数据库、PharmGkb数据库、TTD数据库、DrugBank数据库预测骨质疏松骨折的相关靶点,制作维恩图获取淫羊藿与骨质疏松骨折的共同靶点,运用Cytoscape软件绘制中药-成分-疾病-靶点调控网络,利用STRING数据库进行蛋白相互作用网络的构建与分析。最后,使用R语言和Bioconductor平台进行基因本体富集分析(GO)和基因相互作用通路分析(KEGG)。结果淫羊藿主要是通过23种活性成分、121个作用靶点及155个生物学通路发挥作用治疗骨质疏松骨折。其中主要活性成分为槲皮素、山奈酚和木犀草素;主要核心靶点有AKT1、TP53、Jun、HSP90AA1和CASP3等;关键通路为脂质和动脉粥样硬化信号通路。结论通过网络药理学揭示了淫羊藿通过多成分、多靶点、多通路治疗骨质疏松骨折的作用特点,为进一步的研究和实验工作提供了基础。

Objective To identify the the molecular mechanism of epimedium in the treatment of osteoporotic fracture based on network pharmacology.Methods The main active components and targets of epimedium was screened in the Chinese Medicine Computing System Pharmacology Analysis Platform(TCMSP),and a prediction for osteoporotic fracture targets in GeneCards database,OMIM database,PharmGkb database,TTD database and DrugBank database was performed.The common targets of epimedium and osteoporotic fracture were got by Venn diagrams.Cytoscape3.6.1 was used to construct a Chinese medicine-ingredient-disease-target regulation network,then establish a protein-protein interaction network through the STRING data platform,and fi nally use the R language and Bioconductor platform for gene ontology enrichment analysis and gene interaction(KEGG)pathway analysis.Results The treatment of osteoporotic fracture with epimedium is mainly through 23 active components,193 key targets and 155 biological signaling pathways.The main active constituents of the drug were quercetin,kaempferol and luteolin.The key targets were AKT1,TP53,Jun,HSP90AA1,CASP3,etc.The key pathway was Lipid and atherosclerosis signaling pathway.Conclusion This study shows the action characteristic of epimedium in the treatment of osteoporotic fracture may be achieved by multi-components,multi-targets and multi-biological signaling pathways through network pharmacology,and it provide a scientifi c theoretical basis for the further research and experiments.