2014—2018年江西省食源性疾病患者沙门菌分离株MLST分型及流行特征研究

The multi-locus sequence typing and epidemiological characteristics of Salmonella isolated from patients with foodborne diseases from 2014 to 2018 in Jiangxi Province

目的掌握江西省食源性疾病患者沙门菌分离株优势ST(MLST)型及其时空分布特征、流行趋势,分析血清型与ST型的对应关系。方法以分离自2014—2018年江西省食源性疾病患者的313株沙门菌为研究对象,采用多位点序列分型(MLST)技术对菌株进行基因分型,探讨ST型别的时空分布特征及其与血清型的对应关系。结果313株沙门菌共分为39种ST型,占比最高的是ST34(32.59%),其次是ST11(15.97%)、ST19(11.50%)。每年的优势ST型基本为ST34、ST11、ST19,地区间ST型分布存在差异:上饶市ST型种类最多(20种),其次是抚州市(15种)、景德镇市(14种);南昌市优势ST型别为ST11、新余和鹰潭市优势ST型为ST19,而其他地市的优势ST型均为ST34。313株沙门菌共分为30个血清型,优势血清型为4,[5],12:i:-(30.35%)、肠炎沙门菌(15.97%)、鼠伤寒沙门菌(14.06%)。结论江西省食源性疾病患者沙门菌分离株的ST型别较多,年度流行ST型以ST34、ST11、ST19为主。这三种优势ST型在江西省不同地区间均有分布,但ST型在时空分布上也存在地区差异。

Objective To understand the predominant multi-locus sequence typing types,their temporal and spatial distribution characteristics,and epidemic trends of Salmonella isolated from patients with foodborne diseases in Jiangxi Province,the corresponding relation between the serotypes and sequence types(STs)was analyzed.Methods Three hundred and thirteen isolates from patients with foodborne diseases from 2014 to 2018 in Jiangxi Province were used as the research subjects.Multi-locus sequence typing was used to genotype the strains.The relation between the spatial and temporal distribution characteristics of the STs and serotypes was analyzed.Results The 313 isolates were divided into 39 STs.The dominant STs were ST34(32.59%),ST11(15.97%),and ST19(11.50%).The most common ST types annually were ST34,ST11,and ST19.There were regional differences in the distribution of STs.Shangrao had the most STs(20),followed by Fuzhou(15)and Jingdezhen(14).The dominant STs in Nanchang,Xinyu,and Yingtan were ST11,ST19,and ST19,respectively,whereas the dominant ST type in the other cities was ST34.The 313 Salmonella isolates were classified into 30 serotypes.The dominant serotypes were S.serovars 4[5],12:i:-(30.35%),S.enteritidis(15.97%),and S.typhimurium(14.06%).Conclusion Many STs of Salmonella strains,but mainly ST34,ST11,and ST19,were isolated from patients with foodborne diseases in Jiangxi Province.The three dominant STs were distributed in different regions of Jiangxi Province,but there were also individual differences in the temporal and spatial distribution of the STs.