两色金鸡菊降血脂活性部位筛选研究

Study on the Screening of Hypolipidemic Active Fractions from Coreopsis Tinctoria Nutt.

目的:研究两色金鸡菊降血脂活性部位。方法:采用溶剂法通过降血脂活性筛选确定较佳的提取溶剂,并对提取物精制纯化进行降血脂活性考察。结果:两色金鸡菊水提物在高剂量下能显著降低高血脂小鼠血清中TC和LDL-C含量;水提物精制部位在低剂量下可显著降低血清TC和LDL-C含量;水提物和水提物经大孔吸附树脂30%乙醇洗脱部位高剂量下均可显著降低血清TC和LDL-C蛋白含量。结论:两色金鸡菊的最佳提取溶剂为水,大孔吸附树脂30%乙醇洗脱部位为活性部位。

Objective:To determine the lipid-lowering active site of Coreopsis tinctoria Nutt.Methods:The solvent method was used to screen the optimal extraction solvent for lipid-lowering activity,and the extract was refined and purified for lipid-lowering activity evaluation.Results:The aqueous extract of Coreopsis tinctoria Nutt.can significantly reduce the levels of TC and LDL-C in the serum of hyperlipidemic mice at high doses.The refined fraction of water extract can significantly reduce serum TC and LDL-C content at low doses.Both water extract and the 30%ethanol elution site through macroporous adsorption resin can significantly reduce serum TC and LDL-C content at high doses.Conclusion:The optimal extraction solvent for the two color Chrysanthemum is water,and the 30%ethanol elution site of the macroporous adsorption resin is the active site.