浦北陈皮指纹图谱的建立及其黄酮类成分含量测定

Establishment of Fingerprint of Pubei Chenpi and Determination of Its Flavonoids Content

目的:建立浦北陈皮甲醇提取物的HPLC指纹图谱,测定其橙皮苷、川陈皮素、橘皮素3种黄酮类成分,为浦北陈皮的质量控制及其品质鉴定提供科学的数据支撑。方法:以浦北陈皮为研究对象,采用HPLC法,色谱柱为Thermo C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0 mL·min^(-1),柱温为35℃,检测波长为283 nm、330 nm,建立HPLC指纹图谱,以中药色谱指纹图谱相似度评价系统软件(2012.130723版)进行评价。同时测定浦北陈皮中橙皮苷、川陈皮素、橘皮素3种黄酮类成分含量。结果:建立的浦北陈皮指纹图谱相似度在0.910~0.995;测定的橙皮苷(以干燥品计)、川陈皮素(以干燥品计)、橘皮素(以干燥品计)含量分别为2.598%~5.266%、0.238 8%~0.586 8%、0.177 6%~0.435 8%。结论:通过HPLC建立的浦北陈皮甲醇提取物指纹图谱的相似度较高,可为浦北陈皮质量控制提供依据。同时,本次采集的浦北陈皮样品橙皮苷、川陈皮素、橘皮素3种黄酮类成分含量较高,其含量值都符合2020年版《中国药典》要求,可侧面表明浦北陈皮具有较高的利用价值。

Objective:HPLC fingerprint of methanol extract of Pubei Chenpi was established and hesperidin,nobiletin and tangeretin were determined.It can provide scientific basis for quality control and identification of Pubei Chenpi.Method:Pubei Chenpi was studied by HPLC with Thermo C_(18)(250 mm×4.6 mm,5μm),the mobile phase of acetonitrile-water and gradient elution at a flow rate of 1.0 mL·min^(-1),the column temperature of 35℃,and the detection wavelengths of 283 nm and 330 nm.HPLC fingerprint was established and evaluated by traditional Chinese medicine chromatographic fingerprint similarity evaluation system software(version 2012.130723).At the same time,the contents of hesperidin,nobiletin and tangerine in Pubei Chenpi were determined.Result:The similarity of the fingerprint of Pubei Chenpi was between 0.910 and 0.995,and the content of hesperidin(as dried product),nobiletin(as dried product)and tangerine(as dried product)were 2.598%~5.266%,0.2388%~0.5868%,0.1776%~0.4358%,respectively.Conclusion:The fingerprints of methanol extract of Pubei Chenpi established by HPLC showed high similarity,which can provide a basis for the quality control of Pubei Chenpi.At the same time,the contents of hesperidin,nobiletin and tangerine in Pubei Chenpi samples were higher,and their contents were in accordance with the requirements of Chinese Pharmacopoeia 2020 edition,which indicated that Pubei Chenpi had high utilization value.