基于矿物元素指纹图谱鉴定陈皮产地的方法研究

A Method of Origin Identifying of Citrus Reticulata Blanco Based on the Mineral Element Fingerprint

采用电感耦合等离子体质谱法测定了新会、广西、湖南3地42批陈皮样品中51种矿物元素的含量,运用方差分析和偏最小二乘法-判别分析(PLS-DA)研究了不同产地陈皮中矿物元素的差别,以筛选得到的9种元素为溯源指标,将不同数量级矿物元素含量进行分类和系数缩放后,构建了陈皮中矿物元素的指纹图谱,并基于各产地矿物元素含量的平均值构建了新会、广西、湖南产地陈皮中矿物元素的标准指纹图谱。通过采用SPSS20.0计算18批新会陈皮矿物元素指纹图谱与其标准指纹图谱的相似度,确定新会陈皮矿物元素指纹图谱的相似度阈值为0.941,基于此阈值建立了新会陈皮的鉴别模型。采用24批样品对所建立的鉴别模型进行准确性验证,正确率为91.6%。该方法操作简单直观,满足实验分析要求,可为陈皮的产地溯源提供参考。

To figure out the differences in elemental content of citrus reticulata blanco from different producing areas and establish a rapid method for identifying the origin,42 batches of citrus reticulata blanco sample from 3 origins in Xinhui,Guangxi,Hunan were collected in this study.The contents of 51 mineral elements were determined by inductively coupled plasma-mass spectrometry(ICP-MS).Combined with analysis of variance and partial least squares discriminant analysis(PLS-DA),9 effective indicators were screened out.And the conversion coefficient was studied and the elemental fingerprint was established.The standard fingerprints of Xinhui,Guangxi and Hunan were con-structed based on the average values of element content in each production area,serving as the basis for traceability of production areas.SPSS 20.0 was used to calculate the similarity between the fin-gerprints and the similarity threshold was determined to be 0.941,which was used to distinguish the producing area.Lastly,24 batches of other sample were used to verify the accuracy of the method,with a accuracy rate of 91.6%.The method is simple and intuitive to operate,and can meet the re-quirements of experimental analysis.The established standard fingerprint of citrus reticulata blanco can provide a reference method for the traceability of the origin of tangerine peel.