干血斑中34种苯二氮卓类物质的实时直接分析-串联质谱快速筛查研究

Rapid Screening for 34 Benzodiazepines in Dried Blood Spots Using Direct Analysis in Real Time-Tandem Mass Spectrometry

该文通过优化仪器条件和前处理方法建立了实时直接分析-串联质谱快速筛查干血斑(DBS)中34种苯二氮卓类物质(BZDs)的方法。首先将50μL血液沉积在采血卡上形成DBS,DBS经溶剂充分提取后取上层溶液,使用DART 12Dip-It^(TM)模块进样5μL,在多反应监测模式下检测。结果表明:选用400℃作为载气加热器温度,以乙酸乙酯-水(体积比3∶1)混合溶剂提取DBS样品,所得响应强度较高且建立的筛查方法选择性良好,无延迟效应影响,除阿地唑仑、奥沙西泮和α-羟基三唑仑外,其他物质的检出限均在5~50 ng/mL。所建方法可在90.3%的阳性检材中成功筛选出BZDs物质,方法快速、简便、有效,适用于服药自杀等类案件中DBS检材的快速筛查检验。

In this paper,a rapid and effective screening method for 34 benzodiazepines(BZDs)in dried blood spots(DBS)using direct analysis in real time-tandem mass spectrometry(DART-MS/MS)was established by optimizing the instrumental conditions and pre-treatment methods.50μL of blood was deposited on the blood collection card to form DBS,the upper solution was taken after sufficient solvent extraction and 5μL was injected using the DART 12Dip-It^(TM) module and assayed in multiple reaction monitoring(scheduled MRM)mode.The results showed that:400℃was chosen as the carrier gas heater temperature,and the DBS samples were extracted using a solvent mixture of ethyl acetate-water(3∶1,by volume),and the upper layer of the solution was injected,which showed a high response intensity and good selectivity without the influence of delayed effect.The limits of detection were in the range of 5-50 ng/mL for all substances except for adinazolam,oxazepam andα-hydroxytriazolam.BZDs were successfully screened in 90.3%of the positive case samples.The method is fast,simple,effective and suitable for the rapid screening test of DBS samples in cases such as suicide by drug taking.