感染后肠易激综合征肠道菌群及血清代谢物对内脏敏感性影响

Effects of Intestinal Flora and Serum Metabolites on Visceral Sensitivity in Post-InfectionIrritable Bowel Syndrome

目的:探讨感染后肠易激综合征(PI-IBS)小鼠肠道菌群与血清代谢物的变化,分析其对内脏敏感性的影响。方法:采用旋毛虫感染法制备PI-IBS模型,利用16S rDNA测序分析PI-IBS小鼠和正常小鼠肠道微生物变化;血清代谢组学分析血清代谢物变化;Spearman法对2组的差异代谢物和差异菌群进行关联分析。结果:16S rDNA测序结果显示mouse gut metagenome和g_Alistipes可能是PI-IBS小鼠的关键肠道微生物。代谢组学分析共筛选出22种组间差异代谢物,涉及16条代谢通路,主要包括氨基酸的生物合成、半胱氨酸和蛋氨酸代谢、精氨酸生物合成、突触囊泡循环等通路。结论:肠道菌群和血清代谢物改变参与PI-IBS内脏高敏感性的产生。

Objective:To investigate the changes of intestinal flora and serum metabolites in mice with post-infection irritable bowel syndrome(PI-IBS),and analyze the effects on visceral sensitivity.Methods:The PI-IBS model was prepared by Trichinella spiralis infection method,and the intestinal microbial changes of PI-IBS mice and normal mice were analyzed by 16S rDNA sequencing.The changes of serum metabolites were analyzed by serum metabolomics.Spearman′s correlation analysis was used to analyze the correlation between different metabolites and different flora in the two groups.Results:The results of 16S rDNA sequencing showed that mouse gut metagenome and g_Alistipes were the critical gut microbes in PI-IBS mice.A total of 22 intergroup differential metabolites were identified by metabolomics analysis,involving 16 metabolic pathways,mainly including biosynthesis of amino acids,cysteine and methionine metabolism,arginine biosynthesis,synaptic vesicle cycle and other pathways.Conclusion:Changes in intestinal flora and serum metabolites are involved in the production of visceral hypersensitivity to PI-IBS.