柴胡皂苷D对高尿酸血症大鼠尿酸及相关酶活性的影响

Effects of Saikosaponin D on Uric Acid and Related Enzyme Activity in Rats with Hyperuricemia

目的:探讨柴胡皂苷D对高尿酸血症大鼠体内尿酸水平及相关酶活性的影响。方法:柴胡皂苷D用0.9%氯化钠溶液溶解,配制成5、10、20 mg/mL药液。将50只SD大鼠随机分为对照组、模型组及柴胡皂苷D大、中、小剂量组。除对照组外,其余大鼠每天上午灌胃腺嘌呤0.2 g/kg+乙胺丁醇0.25 g/kg,建立高尿酸血症模型。柴胡皂苷D各剂量组大鼠均以10 mL/kg的剂量灌胃给药,对照组大鼠与模型组大鼠灌胃同等剂量0.9%氯化钠溶液。检测给药前、给药后14、21、28 d大鼠血尿酸、尿尿酸以及黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)、肌酐和尿素氮的水平。结果:给药后21 d,与对照组比较,模型组大鼠血尿酸水平明显升高;与模型组相比,柴胡皂苷D中剂量组血尿酸水平显著下降,差异均具有统计学意义(P<0.05);给药后28 d,与模型组相比,柴胡皂苷D大剂量组、中剂量组血尿酸水平显著下降,中剂量组及小剂量组大鼠尿尿酸水平显著降低;柴胡皂苷D各剂量组大鼠的ADA、XOD、肌酐及尿素氮水平均显著下降,差异均具有统计学意义(P<0.05)。结论:柴胡皂苷D可以通过降低ADA以及XOD水平,抑制尿酸生成,起到降尿酸的作用,具有显著的肾脏保护功能。

Objective: To investigate the effects of saikosaponin D on the level of uric acid and related enzyme activity in rats withhyperuricemia. Methods: Saicosaponin D was dissolved in 0.9% sodium chloride solution to prepare 5, 10, and 20 mg/mL solutions.Fifty SD rats were randomly divided into a control group, a model group, and high, medium and low-dose groups of sausaponin D.Except for the control group, the remaining rats were administrated with adenine 0.2 g/kg + ethambutol 0.25 g/kg every morning toestablish a hyperuricemia model. The rats in the drug groups were administrated intragastrically at a dose of 10 mL/kg, while the rats inthe control and model groups were administrated intragastrically with the same dosage of 0.9% sodium chloride solution. The levels ofserum uric acid, urinary uric acid, xanthine oxidase (XOD), adenosine deaminase (ADA), creatinine and urea nitrogen were detectedbefore administration as well as 14, 21 and 28 days after administration. Results: On the 21st day after administration, the serum uricacid level in the model group rats significantly increased. Compared with the model group, the serum uric acid level of rats in themedium-dose group of saikosaponin D significantly decreased with statistical significance (P<0.05). On the 28th day afteradministration, the serum uric acid level of rats in the high-dose and medium-dose groups significantly decreased, and the urinary uricacid level of rats in the medium-dose and low-dose groups also decreased significantly. The levels of ADA、XOD、creatinine and ureanitrogen in all rats of each measurement group of chainu sausaponin D were significantly decreased, and the differences werestatistically significant (P<0.05). Conclusion: Saikosaponin D can inhibit the production of uric acid by reducing the levels of ADAand XOD, and play a role in lowering uric acid, which has significant renal protective function.