基于全基因组重测序对ARTP诱变的一株高产淀粉酶韩国普李斯特氏菌的关键基因研究

A study on key gene in an efficient amylase productionstrain of Priestia koreensis by ARTP mutationbased on whole genome resequencing

为提高一株海洋来源的韩国普李斯特氏菌(Priestia koreensis FS-1)产淀粉酶的能力,对该株菌采用常压室温等离子体(atmospheric and room temperature plasma, ARTP)技术进行了诱变选育。结果显示:经过诱变选育后得到一株产淀粉酶活力较原始菌株提高了90%且稳定遗传的突变菌株P.koreensis FS-103。为解析该突变菌株酶活力增加的分子机制,对突变菌株P.koreensis FS-103进行了全基因组重测序及比对分析研究。相关分析显示:首先,P.koreensis FS-103的基因组中乙酰化修饰、抗氧化作用以及同义突变相关的基因发生了遗传变异;其次,对COG(clusters of orthologous groups)功能和变异基因进行富集分析,P.koreensis FS-103转录、翻译和翻译后修饰过程相关基因均上调。经过ARTP诱变以后的突变菌株P.koreensis FS-103淀粉酶活力提升的原因是以上功能基因发生上调效应。

In this study,Atmospheric Room Temperature Plasma(ARTP)technique was used to screen a original strain of amylase-producing Priestia koreensis FS-1.Results showed that a mutanted P.koreensis FS-103 with improved enzyme activity and stable genetics was obtained by the technique and its amylase activity was 90%higher than that of the original strain.To analyze the molecular mechanism that increase the enzyme activity of the screened strain,whole genome resequencing and comparative analysis was used to study the mutanted strain P.koreensis FS-103.Results show that the genetic variations in acetylation,antioxidant activity and synonymous mutation were found in P.koreensis FS-103.While the up-rugulation of differential gene expression involved in transcription,translation and post-translation modifying process were found in the functionalenrichment analysis of COG(clusters of orthologous groups)and the varianted genetics.As conclusions,it indicates that the up-regulations of the serial functuional expression gene of the mutanted strain P.koreensis FS-103 are the key reason for the improved enzyme activity.