北苍术与朝鲜苍术健脾作用及机制研究

Study on spleen strengthening effects and mechanisms of Atractylodeschinensis and Atractylodes coreana

目的 分析北苍术与朝鲜苍术干预后,脾虚大鼠血浆生物标志物及代谢通路的差异,从非靶向代谢组学的角度讨论两者的健脾机制。方法 将SD大鼠采用饮食不节、疲劳过度加苦寒泻下的复合因素法,造成脾虚症模型。测定胃肠道和免疫学指标的水平;采用超高效液相色谱串联Q Exactive质谱仪(UHPLC-QE-MS)技术,联合主成分分析(PCA)与正交偏最小二乘法判别分析(OPLS-DA)方法,寻找脾虚大鼠血浆中生物标志物;利用Pathway数据库归纳代谢通路。结果 经北苍术与朝鲜苍术给药后,脾虚大鼠血浆中各指标均有不同程度回调。代谢组学分析表明,北苍术与朝鲜苍术分别回调了70个和82个血浆差异代谢物,北苍术主要调节“甘氨酸、丝氨酸和苏氨酸代谢”与“硫胺素代谢”2个代谢通路,朝鲜苍术主要调节“甘氨酸、丝氨酸和苏氨酸代谢”、“硫胺素代谢”、“嘧啶代谢”、“丁酸代谢”与“核黄素代谢”5个代谢通路。结论 北苍术与朝鲜苍术对脾虚大鼠均具有一定的调节作用,其作用机制可能与调节“甘氨酸、丝氨酸和苏氨酸代谢”、“硫胺素代谢”等代谢通路有关。

Aim To analyze the differences in plasma biomarkers and metabolic pathways between Atractylodes chinensis and Atractylodes coreana after intervention in spleen deficiency rats,and discuss the spleen strengthening mechanism of the two from a non targeted metabolomics perspective.Methods A spleen deficiency model was established in SD rats using a composite factor method of improper diet,excessive fatigue,and bitter cold diarrhea.To determine the content of gastrointestinal and immunological indicators,UHPLC-QE-MS technology was used,combined with principal component analysis(PCA)and orthogonal projections to latent structures-discriminant analysis(OPLS-DA)methods to search for biomarkers in plasma of spleen deficiency rats,and metabolic pathways were induced using the Pathway database.Results After administration of Atractylodes chinensis and Atractylodes coreana,various indicators in plasma of spleen deficiency rats showed varying degrees of regression.Metabolomics analysis showed that Atractylodes chinensis and Atractylodes coreana respectively recalled 70 and 82 plasma differential metabolites.Atractylodes chinensis mainly regulated two metabolic pathways:“Glycine,serine,and threonine metabolism”and“Thiamine metabolism”.Atractylodes coreana mainly regulated five metabolic pathways:“Glycine,serine,and threonine metabolism”,“Thiamine metabolism”,“Pyrimidine metabolism”,“Butanoate metabolism”,and“Riboflavin metabolism”.Conclusions Both Atractylodes chinensis and Atractylodes coreana have certain regulatory effects on spleen deficiency rats,and their mechanism of action may be related to regulating metabolic pathways such as“Glycine,serine,and threonine metabolism”and“Thiamine metabolism”in spleen deficiency.