摘要
目的:建立亚玛熏药散中木香烃内酯的含量测定方法。方法:采用高效液相色谱法,色谱柱为ZORBOX SB-C_(18) 5μm 4.6 mm×250 mm;流动相为乙腈∶水(50∶50)洗脱;流速:0.8 mL/min;柱温:35℃;检测波长:225 nm。结果:木香烃内酯在8.59~85.91μg/mL的浓度范围内线性关系良好,r=0.9999,回收率为97.4%。结论:该方法经方法学验证准确可行,可用于亚玛熏药散的质量控制。
Objective Aim to establish a method for the deter mination of Costunolide in Yama fumigation powder.Methods High performance liquid chromatography was used. The column was ZORBOX SB-C_(18) 5 μm 4.6 mm×250 mm, the mobile phase was acetonitrile∶water(50∶50), the flow rate was 0.8 mL/min, the column temperature was 35 ℃, and the detection wavelength was 225 nm. Results the linear relationship of Costunolide was good in the range of 8.59-85.91 μg/mL,r=0.9999, the recovery rate was 97.4%.Conclusion The method is proved to be accurate and feasible by methodology, and can be used for the quality control of Yama fumigation powder.
作者
郭司群
鲁海涛
迟晓雪
郭刚
魏红
GUO Siqun;LU Haitao;CHI Xiaoxue;GUO Gang;WEI Hong(Hulunbeier Institute for Food and Drug Control,Hulunbeier 021008,China;Hulunbuir Institute of Forestry and Grassland Science,Hulunbeier 021008,China;Hulunbeier Center for Disease Control and Prevention Inner Mongolia,Hulunbeier 021008,China)
出处
《中国民族民间医药》
2024年第3期33-36,共4页
Chinese Journal of Ethnomedicine and Ethnopharmacy