麝香草酚与浓盐酸保存尿液检测24h尿枸橼酸、草酸、磷酸结果的差异

Comparison of measurement results of citric acid,oxalic acid,and phosphoric acid in 24-hour urine preserved with thymol and concentrated hydrochloric acid

目的比较麝香草酚与浓盐酸保存尿液检测24h尿枸橼酸、草酸和磷酸结果的差异。方法收集2023年7月至2023年8月于郑州大学第二附属医院就诊的50例上尿路结石患者的尿液标本。男38例,女12例;年龄(45.52±13.68)岁。患者人院第2天晨起排空膀胱,收集24h尿液。将每例患者的尿液样本均分为两份,存放于密封塑料容器中,并分别加入浓盐酸(15ml,浓度为6 mmol/L)和麝香草酚(2g)作为防腐剂,分别为浓盐酸组和麝香草酚组。检测两组尿液中草酸、枸橼酸和磷酸的浓度,并将检测值乘以尿液总量得出24h尿液中草酸、枸橼酸和磷酸含量的最终检测结果。比较两组24h尿液枸橼酸、草酸、磷酸检测结果的差异。结果在浓盐酸组中,24h尿液枸橼酸中位值为160.87(95.37,245.61)mg/24h,草酸中位值为33.09(26.32,39.37)mg/24h,磷酸为(731.06±240.62)mg/24h。在麝香草酚组中,枸橼酸中位值为340.40(166.56,561.77)mg/24h,草酸中位值为33.91(26.51,44.56)mg/24h,磷酸为(759.07±273.23)mg/24h。与麝香草酚组相比,浓盐酸组枸橼酸检测结果显著降低(Z=4.31,P<0.001);两组草酸(Z=0.85,P=0.393)、磷酸(t=1.65,P=0.106)检测结果差异无统计学意义。结论与麝香草酚相比,以浓盐酸为防腐剂的24h尿液枸橼酸测量结果偏低,草酸、磷酸测量结果无明显差异。在行24h尿液代谢分析检测枸橼酸、草酸和磷酸时,采用麝香草酚作为防腐剂较浓盐酸更适合。

Objective To compare the differences in the results of 24-hour urine citric acid,oxalic acid,and phosphoric acid detection between thymol and concentrated hydrochloric acid preserved urine.Methods Urine samples were collected from 50 patients with upper urinary tract stones who were admitted to the Second Affiliated Hospital of Zhengzhou University from July 2023 to August 2023.There were 38 males and 12 females,with the age of(45.52±13.68)years old.On the second day of admission,patients were required to empty their bladders in the morning and collect urine over a 24-hour period.The urine was evenly divided and stored in two sealed plastic containers.In one container,concentrated hydrochloric acid(15 ml,concentration of 6 mmol/L)was added as a preservative,forming the hydrochloric acid group.In the other container,2 g of thymol was added as a preservative,forming the thymol group.The concentration of oxalic acid,citric acid,and phosphoric acid in urine were measured,and the measurement results were multiplied by the total amount of urine to obtain the final measurement results.The differences in the measurement results of citric acid,oxalic acid,and phosphoric acid between two groups were compared.Results In the concentrated hydrochloric acid group,the median value of citric acid was 160.87(95.37,245.61)mg/24h,the median value of oxalic acid was 33.09(26.32,39.37)mg/24h,and the mean value of phosphoric acid was(731.06±240.62)mg/24h.In the thymol group,the median value of citric acid was 340.40(166.56,561.77)mg/24h,the median value of oxalic acid was 33.91(26.51,44.56)mg/24h,and the mean value of phosphoric acid was(759.07±273.23)mg/24h.Compared with the thymol group,the citric acid level in the concentrated hydrochloric acid group was significantly lower(Z=4.31,P<0.001),but there was no significant difference in oxalic acid(Z=0.85,P=0.393)and phosphoric acid(t=1.65,P=0.106)between the two groups..Conclusions Compared with thymol,the results of 24 h urine citric acid with concentrated hydrochloric acid as preservative is lower,but there is no significant difference between oxalic acid and phosphoric acid.The use of thymol as a preservative may be more suitable than concentrated hydrochloric acid for the detection of citric acid,oxalic acid and phosphoric acid in 24-hour urine metabolism analysis.