类器官模型在肝细胞癌中的研究进展

A Review of Organoids as Research Models for Hepatocellular Carcinoma

类器官作为一种维持自身组织结构和功能的体外3D模型,很大程度上克服了传统肿瘤模型(包括2D细胞培养和体内小鼠培养)的局限性,近年来已成为研究肿瘤领域的有力工具。肝细胞癌(HCC)类器官从人和鼠来源的组织样品中产生,保留了亲本肿瘤组织的关键特征,包括组织学结构、基因组景观、表达谱和肿瘤内异质性。目前类器官在乙型肝炎病毒(HBV)及其他因素诱导的肝癌机制探索、药物筛选、个体化医疗中的应用正在被广泛研究。文章就类器官的起源、特性及其在肝癌中的应用展开综述。

Organoids,as in vitro 3D models which maintain their own tissue structure and function,have largely overcome the limitations of traditionally-cultured tumor models,including 2D cell culture and in vivo mouse culture,and organoid culture has become a powerful tool in tumor research in recent years.Hepatocellular carcinoma(HCC)organoids are generated from tissue samples of human and mouse origin and retain key features of parental tumor tissues,including histological structure,genomic landscape,expression profile,and intratumoral heterogeneity.The application of organoids in exploring the mechanism of hepatitis B virus(HBV)and other factor-induced hepatocellular carcinoma,drug screening,and individualized medical treatment is currently being widely investigated.This article provides a review on the origin and characterization of organoids and their application in hepatocellular carcinoma.