14种喀斯特地区植物药提取物的体外抗氧化活性比较

Comparison of in vitro antioxidant activity of 14 phytomedicine extracts in Karst areas

目的 对14种喀斯特地区特色植物药抗氧化活性进行筛选,为氧化应激诱导的相关疾病药物干预提供基础数据。方法 构建抗氧化测定体系(清除羟自由基能力的测定、清除2,2-联苯基-1-苦基肼基(DPPH)自由基能力的测定、铁氰化钾还原能力的测定及抑制脂质过氧化能力测定),以维生素C作为阳性对照,检测14植物药醇提取物的体外抗氧化活性,采用隶属函数分析法进行结果的综合评价及排序。结果 植物药对羟自由基的清除能力强弱为仙桃草、瓜子金>凉粉柴>天冬>锦茵陈>维生素C>其余药物;植物药对DPPH自由基的清除能力强弱为维生素C>锁阳>吴茱萸>响铃草>皂角>薏苡仁>仙桃草>其余药物;植物药还原能力的强弱为吴茱萸、仙桃草、锁阳、锦茵陈和菟丝子的还原能力比阳性对照组强,响铃草和皂角的还原能力与对照组相近,其余药物弱于对照组;植物药抑制脂质过氧化能力强弱为凉粉柴>仙桃草>响铃草>吴茱萸>菟丝子>玉竹>瓜子金>天冬>维生素C>其余药物;最后通过隶属函数分析显示,14种植物药的抗氧化能力为仙桃草>吴茱萸>维生素C>凉粉柴>响铃草>锁阳>天冬>皂角>瓜子金>薏苡仁>菟丝子>锦茵陈>黄精>一朵云>玉竹。结论 所检测的植物药中,仙桃草可能作为潜在的抗氧化应激候选药物;采用抗氧化评价体系结合隶属函数分析,更加科学可靠地评价了药物的抗氧化能力。

Objective To screen the antioxidant activities of 14 phytomedicines unique to the Karst areas,providing foundational data for pharmacological intervention in diseases induced by oxidative stress.Methods An antioxidant assay system was established,including assays for hydroxyl radical scavenging,DPPH radical scavenging,potassium ferricyanide reducing power,and lipid peroxidation inhibition.The in vitro antioxidant activities of alcoholic extracts from 14 phytomedicines were evaluated,with Vitamin C as the positive control.Comprehensive assessment and ranking of results were conducted using the membership function analysis method.Results The efficacy of phytomedicines in hydroxyl radical scavenging,in descending order,was as follows:Veronica peregrina,Polygala japonica houtt>Premna microphylla turcz>Asparagus cochinchinensis.(Lour.)Merr.>Artemisia capillaries>Vitamin C>other phytomedicines.For DPPH radical scavenging,the order was:Vitamin C>Cynomorium songaricum>Euodiae fructus>Crotalaria ferruginea>Gleditsia sinensis>Coicis semen>Veronica peregrina>other phytomedicines.Regarding reducing power,Euodiae fructus,Veronica peregrina,Cynomorium songaricum,Artemisia capillaries,and Semen cuscutae Chinensis exhibited stronger activity than the positive control,while Crotalaria ferruginea and Gleditsia sinensis were similar to the control,and the remaining phytomedicines were weaker.In lipid peroxidation inhibition,the order was:Premna microphylla turcz>Veronica peregrina>Crotalaria ferruginea>Euodiae fructus>Semen cuscutae>Polygonatum odoratum>Polygala japonica houtt>Asparagus cochinchinensis>Vitamin C>other phytomedicines.The membership function analysis revealed the antioxidant capacities of the 14 phytomedicines as follows:Veronica peregrina>Euodiae fructus>Vitamin C>Premna microphylla turcz>Crotalaria ferruginea>Cynomorium songaricum>Asparagus cochinchinensis>Gleditsia sinensis>Polygala japonica houtt>Coicis semen>Semen cuscutae>Artemisia capillaries>Polygonati rhizoma>Botrychium ternatum(Thunb.)Sw.>Polygonatum odoratum.Conclusion Among the tested phytomedicines,Veronica peregrina emerges as a potential antioxidant stress candidate.The antioxidant evaluation system combined with membership function analysis provide a more scientific and reliable assessment of the antioxidant capacities of these phytomedicines.