多囊卵巢综合征患者血清miR-23a、miR-143表达及与胰岛素抵抗的相关性分析

Analysis of serum miR-23a and miR-143 expression and correlation with insulin resistance in patients with polycystic ovary syndrome

目的:探讨多囊卵巢综合征(PCOS)患者血清微小RNA-23a(miR-23a)、微小RNA-143(miR-143)表达及其与胰岛素抵抗的相关性分析。方法:选取2022年1月至2023年6月枣庄市妇幼保健院收治的68例PCOS患者(PCOS组)和50例健康体检女性(对照组)为研究对象,均采用实时荧光定量PCR(qRT-PCR)检测血清miR-23a、miR-143表达,PCOS患者根据胰岛素抵抗指数(HOMA-IR)分为胰岛素抵抗组(HOMA-IR≥2.69)40例和非胰岛素抵抗组(HOMA-IR<2.69)28例,比较各组血清miR-23a、miR-143表达水平,并采用Pearson相关分析血清miR-23a、miR-143表达与胰岛素抵抗的相关性。结果:PCOS组血清miR-23a、miR-143表达水平均高于对照组(P<0.05)。胰岛素抵抗组PCOS患者血清miR-23a、miR-143表达水平均高于非胰岛素抵抗组(P<0.05)。Pearson相关分析显示,PCOS患者血清miR-23a、miR-143表达水平与HOMA-IR均呈正相关(P<0.05)。结论:PCOS患者血清miR-23a、miR-143均呈异常高表达,且表达水平与HOMA-IR有关,可作为PCOS病情评估的有效生物学标志物。

Objective:To investigate the expression of serum micro-RNA-23a(miR-23a)and micro-RNA-143(miR-143)in patients with polycystic ovary syndrome(PCOS)and its correlation with insulin resistance.Methods:68 PCOS patients(PCOS group)and 50 women(control group)admitted to Maternity and Child Health Care Hospital of Zaozhuang from January 2022 to June 2023 were selected as study subjects.Real-time fluorescence quantitative PCR(qRT-PCR)was used to test serum miR-23a and miR-143 expression.PCOS patients were divided into insulin resistance groups(HOMA-IR≥2.69)and 28 cases in non-insulin resistance groups(HOMA-IR<2.69)according to the insulin resistance index(HOMA-IR≥2.69)and 28 cases in the non-insulin resistance group(HOMA-IR<2.69)were compared.Serum miR-23a and miR-143 expression levels were used,and the correlation between serum miR-23a and miR-143 expression and insulin resistance was analyzed using Pearson correlation.Results:The expression levels of serum miR-23a and miR-143 in PCOS group were higher than those in control group(P<0.05).The expression levels of serum miR-23a and miR-143 in PCOS patients in insulin resistance group were higher than those in non-insulin resistance group(P<0.05).Pearson correlation analysis showed that the expression levels of serum miR-23a and miR-143 were positively correlated with HOMA-IR in PCOS patients(P<0.05).Conclusion:The serum miR-23a and miR-143 of PCOS patients are all abnormally highly expressed,and the expression levels are related to HOMA-IR,which can be used as effective biological markers for the evaluation of PCOS conditions.