HPLC-VWD-ELSD法同时测定消炎宁胶囊中7个成分的含量

Simultaneous Determination of Seven Components in XiaoYanNing Capsules by HPLC-VWD-ELSD

目的 采用高效液相色谱-紫外检测器-蒸发光散射检测器(HPLC-VWD-ELSD)法同时测定消炎宁胶囊中苦玄参苷IA、异嗪皮啶、迷迭香酸、金丝桃苷、甘草苷、冬青素A和毛冬青皂苷A1的含量。方法 采用Waters Symmetry C_(18)色谱柱(250 mm×4.6 mm, 5μm),流动相为乙腈(A)-0.15%醋酸水溶液(B),梯度洗脱,流速为1.0 mL·min^(-1)。苦玄参苷IA、异嗪皮啶、迷迭香酸、金丝桃苷、甘草苷采用VWD进行检测,切换波长分别为270 nm及342 nm;冬青素A和毛冬青皂苷A1采用ELSD进行检测,漂移管温度为85℃,载气流速3.5 L·min^(-1)。结果 苦玄参苷IA、异嗪皮啶、迷迭香酸、金丝桃苷、甘草苷、冬青素A和毛冬青皂苷A1进样量分别在1.764~35.28μg、0.6903~13.81μg、0.9524~19.05μg、0.4067~8.134μg、0.2136~4.272μg、0.9182~18.36μg和0.7665~15.33μg范围内与峰面积呈良好的线性关系(r≥0.9991),平均回收率及RSD分别为100.01%(0.81%)、99.14%(0.84%)、99.40%(0.92%)、99.34%(1.02%)、99.20%(1.04%)、98.83%(1.09%)、98.99%(1.08%)。4批消炎宁胶囊中上述7个成分的含量测定结果分别为6.812~6.901 mg·g^(-1)、1.726~1.789 mg·g^(-1)、2.391~2.422 mg·g^(-1)、1.134~1.155 mg·g^(-1)、0.6087~0.6105 mg·g^(-1)、2.306~2.324 mg·g^(-1)和1.995~2.016 mg·g^(-1)。结论 所建立的方法简捷,重复性好,可用于消炎宁胶囊中7个成分的含量测定,为消炎宁胶囊全面的质量控制提供科学依据。

OBJECTIVE To develop a method of high performance liquid chromatography-variable wavelength detector-evaporative light scattering detection(HPLC-VWD-ELSD)for simultaneous determination of picfeltarraenin IA,isofraxidin,rosmarinic acid,hyperoside,liquiritin,ilexgenin A chlorogenic acids and ilexsaponin A 1 in XiaoYanNing Capsules.METHODS HPLC assay was performed on a Waters Symmetry-C_(18) column(250 mm×4.6 mm,5μm)with a mixture of acetonitrilel and 0.15%aqueous acetic acid as the mobile phase in gradient elution at the flow rate of 1.0 mL·min^(-1).Detection wavelength for picfeltarraenin IA,isofraxidin,rosmarinic acid,hyperoside,liquiritin was set at 270 nm and 342 nm;ELSD was used for detection ilexgenin A chlorogenic acids and ilexsaponin A 1.The temperature of the drift tube was maintained at 85℃and the nebulizing gas flow rate was 3.5 L·min^(-1).RESULTS Picfeltarraenin IA,isofraxidin,rosmarinic acid,hyperoside,liquiritin,ilexgenin A chlorogenic acids and ilexsaponin A 1 showed good linearity in the ranges of 1.764-35.28μg,0.6903-13.81μg,0.9524-19.05μg,0.4067-8.134μg,0.2136-4.272μg,0.9182-18.36μg and 0.7665-15.33μg(r≥0.9991),the average recoveries and RSD were 100.01%(0.81%),99.14%(0.84%),99.40%(0.92%),99.34%(1.02%),99.20%(1.04%),98.83%(1.09%),98.99%(1.08%)respectively.The contents of the above mentioned components in four samples were 6.812-6.901 mg·g^(-1),1.726-1.789 mg·g^(-1),2.391-2.422 mg·g^(-1),1.134-1.155 mg·g^(-1),0.6087-0.6105 mg·g^(-1),2.306-2.324 mg·g^(-1) and 1.995-2.016 mg·g^(-1),respectively.CONCLUSION The method is simple and reproducible.It can be used for the determination of the seven components in XiaoYanNing Capsules,providing a scientific basis for the comprehensive quality control of XiaoYanNing Capsules.