ITGB1和CANT1在肺癌组织中的表达及其与临床预后的关系

The expression of ITGB1 and CANT1 in lung cancer tissue and their relationship with clinical prognosis

目的探讨整合素β1(ITGB1)和钙激活核苷酸酶1(CANT1)在肺癌组织中的表达及其与临床预后的关系。方法选取2018年4月~2020年5月期间在本院住院并行手术治疗的78例肺癌患者的肺癌组织及距离癌组织3cm处的癌旁组织为研究对象,采用免疫组织化学法检测肺癌组织及癌旁组织中ITGB1、CANT1蛋白表达情况;肺癌组织中ITGB1、CANT1蛋白表达的相关性采用Spearman相关分析;采用Kaplan-Meier法分析肺癌患者肺癌组织中ITGB1、CANT1蛋白阳性表达与生存率的关系;采用Cox回归分析影响肺癌患者预后的因素。结果肺癌组织中ITGB1、CANT1蛋白表达阳性率高于癌旁组织,差异有统计学意义(P<0.05);肺癌组织中ITGB1、CANT1蛋白表达水平与肺癌患者淋巴结转移、组织分化程度、TNM分期有关(P<0.05);肺癌组织中ITGB1、CANT1蛋白表达呈正相关(r s=0.507,P<0.001)。肺癌患者3年生存率为51.28%(40/78)。肺癌组织中ITGB1蛋白阳性表达患者3年生存率(15/48,31.25%)低于ITGB1蛋白阴性表达患者(25/30,83.33%),差异具有统计学意义(χ^(2)=21.986,P<0.001);肺癌组织中CANT1蛋白表达阳性患者3年生存率(16/50,32.00%)低于CANT1蛋白表达阴性患者(24/28,85.71%),差异具有统计学意义(χ^(2)=19.543,P<0.001)。多因素Cox回归分析显示,组织低分化、TNM分期处于Ⅲ-Ⅳ期、有淋巴结转移、ITGB1蛋白阳性表达、CANT1蛋白阳性表达是肺癌患者3年内死亡的危险因素(P<0.05)。结论肺癌组织中ITGB1、CANT1蛋白表达阳性率高于癌旁组织,ITGB1、CANT1蛋白表达可影响肺癌患者预后。

Objective To explore the expression of integrinβ1(ITGB1)and calcium-activated nucleotidase 1(CANT1)in lung cancer and their relationship with clinical prognosis.Methods From April 2018 to May 2020,78 lung cancer patients who were hospitalized and underwent surgical treatment in our hospital were collected as the study subjects,including lung cancer tissue and adjacent tissue located 3 cm away from the cancer tissue,an immunohistochemical method was applied to detect the expression of ITGB1 and CANT1 proteins in lung cancer tissues and adjacent tissues;the correlation between the expression of ITGB1 and CANT1 proteins in lung cancer tissue was analyzed using Spearman correlation analysis;Kaplan-Meier method was applied to analyze the relationship between the positive expression of ITGB1 and CANT1 proteins and survival rate in lung cancer tissues of lung cancer patients;COX regression was applied to analyze factors that affected the prognosis of lung cancer patients.Results The positive rates of ITGB1 and CANT1 protein expression in lung cancer tissues were higher than those in adjacent tissues(P<0.05);the expression levels of ITGB1 and CANT1 proteins in lung cancer tissue were related to lymph node metastasis,tissue differentiation,and TNM staging in lung cancer patients(P<0.05);the expression of ITGB1 and CANT1 proteins in lung cancer tissue was positively correlated(r s=0.507,P<0.001).The 3-year survival rate of lung cancer patients was 51.28%(40/78).The 3-year survival rate(15/48,31.25%)of patients with positive expression of ITGB1 protein in lung cancer tissue was lower than that of patients with negative expression of ITGB1 protein(25/30,83.33%)(χ^(2)=21.986,P<0.001);the 3-year survival rate of patients with positive CANT1 protein expression(16/50,32.00%)in lung cancer tissue was lower than that of patients with negative CANT1 protein expression(24/28,85.71%)(χ^(2)=19.543,P<0.001).Multivariate Cox regression analysis showed that low tissue differentiation,TNM stageⅢ-Ⅳ,lymph node metastasis,positive expression of ITGB1 protein,and positive expression of CANT1 protein were risk factors for 3-year death in lung cancer patients(P<0.05).Conclusion The positive expression rates of ITGB1 and CANT1 proteins in lung cancer tissues are higher than those in adjacent tissues.The expression of ITGB1 and CANT1 proteins can affect the prognosis of lung cancer patients.