人参酵素皂苷含量的分析研究

Analysis and Study on the Content of Ginseng Enzyme Saponins

人参酵素是以人参作为主要原料,经过发酵产生的一种红棕色的液体,不但可以预防多种疾病,而且在美容方面发挥着非常重要的作用。人参是营养价值极高的一种植物资源,具有非常强大的滋补能力,研究发现,人参中可以分离出多种皂苷和多糖,它们是人参生理活性的物质基础。文章主要研究人参酵素中皂苷含量,分别使用p H试纸和p H计测定人参酵素的酸碱度;使用分光光度计在400~700 nm波长之间对人参皂苷Re进行光谱分析,得到550 nm是其最大吸收波长,然后在550 nm的波长下以人参皂苷Re作为标准品测定其吸光度,再计算人参酵素中的皂苷含量;pH计测出人参酵素呈酸性,pH为3.68;人参总皂苷在0~100 uL范围内线性关系相对良好,S_(RSD)(相对标准差)为2.1%(n=3),结果显示人参酵素中皂苷含量为0.2 mg/mL。由以上实验结果可知,酵素是一种酸性的液体,易于保存。人参皂苷是人参酵素中的主要活性物质,保留了人参的生物活性。

Ginseng enzyme is a reddish brown liquid produced by fermentation using ginseng as the main raw material.This not only prevents many diseases,but also plays a very important role in beauty.Ginseng is a plant resource with extremely high nutritional value and strong nourishing ability.Through research,it has been found that various saponins and polysaccharides can be separated from ginseng,which is the material basis for the physiological activity of ginseng.The main focus of the project is to study the saponin content in ginseng enzymes.The paper uses pH test strips and pH meters to determine the acidity and alkalinity of ginseng enzymes;Spectral analysis of ginsenoside Re was performed using a spectrophotometer between 400-700 nm wavelength,and 550 nm was obtained as its maximum absorption wavelength.Then,the absorbance value of ginsenoside Re was measured using it as a standard substance at 550 nm wavelength,and the saponin content in ginseng enzyme was calculated;The pH meter measured that the ginseng enzyme was acidic,with a pH value of 3.68;The linear relationship of total ginsenosides is relatively good within the range of 0-100 uL,with an SRSD(relative standard deviation)of 2.1%(n=3).The result shows that the saponin content in ginseng enzyme is 0.2 mg/mL.From the above experimental results,it can be seen that enzymes are acidic liquids that are easy to preserve.Ginsenosides are the main active substances in ginseng enzymes,retaining the biological activity of ginseng.