运动发酵单胞菌鼓风干燥工艺

Air-blast Drying Technology of Zymomonas Mobili

试验旨在研究运动发酵单胞菌(Zymomonas mobilis)鼓风干燥保护剂的添加。本试验以细菌总数和活菌数为指标,采用固态培养基培养运动发酵单胞菌,然后用鼓风干燥的方式对运动发酵单胞菌进行干燥处理,最后使用25%葡萄糖培养基进行发酵,以失重方式评估其活性。结果显示,培养的细菌数达到1.85×10^(10)CFU/g。用固态培养基等质量的乳糖+5%的甘露糖、+2%或+4%的甘油作为组合保护剂,在39℃鼓风干燥过程中细菌数达到1.31×10^(10)CFU/g和1.2×10^(10)CFU/g,且存活率超过94%。干燥前后的失重结果基本一致,表明鼓风干燥后的细菌具有较高活性。

The aim of this experiment was to study the addition of protective agent for air-blast drying of Zymomonas mobilis.In this experiment,the total number of bacteria and the number of viable bacteria were taken as the index,and the Zymomonas mobilis was cultured in solid medium,and then dried by air-blast drying.Finally,fermentation was performed using 25%glucose medium to evaluate its activity in a weight loss manner.The number of bacteria cultured reached 1.85×10^(10) CFU/g.Using solid medium equal quality lactose+5%mannose,+2%or+4%glycerol as a combination protective agent,the number of bacteria reached 1.31×10^(10) CFU/g and 1.2×10^(10) CFU/g during the drying process at 39℃,and the survival rate was more than 94%.The weight loss results before and after drying were basically the same,indicating that the bacteria had higher activity after air-blast drying.