复合益生菌粉改善小鼠肠道菌群的研究

Study on the Improvement of Mouse Intestinal Microflora by Composite Probiotic Powder

为了探究复合益生菌粉及组成该粉剂的原料和辅料分别对小鼠肠道菌群的改善作用,本实验用复合益生菌粉的低、中、高剂量组以及原料组和辅料组分别灌胃雄性SPF级Babl/c小鼠30 d,每周对小鼠进行称重,分别于0 d、14 d和30 d收集小鼠的粪便,对粪便中培养的肠道菌群(肠球菌、肠杆菌、产气荚膜梭菌、乳杆菌以及双歧杆菌)进行计数。实验期间所有小鼠的精神状态、活动、摄食、排泌等均未见明显异常,体重也都在持续增长。实验前后小鼠自身及其组间肠道菌群的研究结果显示,复合益生菌粉剂量组干预前后自身肠道菌群的差异较为显著,且干预后与阴性对照组相比也存在显著差异,主要表现为:小鼠粪便中的双歧杆菌和乳杆菌明显增加,效果优于原料组和辅料组;肠杆菌、肠球菌和产气荚膜梭菌明显减少,抑制有害菌生长的效果优于原料组和辅料组。综上所述,复合益生菌粉有助于改善小鼠的肠道菌群功能,同时复合益生菌粉对肠道菌群的改善效果优于单独的原料组和辅料组。

In order to investigate the improvement effects of composite probiotic powder and the raw materials and excipients that make up the powder on the intestinal microbiota of mice,this experiment used low,medium,and high dose groups of composite probiotic powder,as well as the raw materials and excipients groups,to orally administer male SPF grade Babl/c mice for 30 days.Weekly weight measurements were conducted on mice,and feces were collected on day 0,day 14,and day 30,respectively.The gut microbiota(Enterococcus,Enterobacterium,Clostridium perfringens,Lactobacillus,and Bifidobacterium)in the feces were cultured and counted.During the experiment,there were no significant abnormalities in the mental state,activity,feeding,and excretion of all mice,and their weight continued to increase.The research results of the gut microbiota before and after the experiment showed that there was a significant difference in the gut microbiota of the composite probiotic powder group before and after intervention,and there was also a significant difference between the intervention group and the negative control group.This is mainly manifested as a significant increase in Bifidobacteria and Lactobacilli in mouse feces,with better effects than the raw material group and auxiliary material group;Enterobacterium,Enterococcus,and Clostridium perfringens were significantly reduced,and their inhibitory effect on the growth of harmful bacteria was better than that of the raw material group and auxiliary material group.In summary,composite probiotic powder helps to improve the function of mouse gut microbiota,and the improvement effect of composite probiotic powder on gut microbiota is better than that of the individual raw material group and excipient group.