275 nm波长UVC光的皮肤安全性研究

Research on Skin Safety of UVC Light with 275 nm Wavelength

目的探索275 nm C波段紫外线(Ultraviolet C,UVC)光照射对皮肤细胞和血管内皮细胞生存、生长的影响,以及其长期和短期照射对裸鼠皮肤的影响,以确定其安全性。方法设计275 nm UVC-LED光源,分别对人类永生化表皮细胞和人脐静脉内皮细胞进行不同时间的照射,观察不同照射剂量对其生存生长的影响;对裸鼠皮肤进行不同时间的照射,观察外观变化,并收集照射区域组织进行免疫组化分析。结果低剂量275 nm UVC光直接照射细胞时,有一定的皮肤相关细胞安全性。但是高剂量的275 nm UVC光照射会对细胞产生较大伤害。同时,不论是低剂量还是高剂量照射275 nm UVC光均不会诱导裸鼠皮肤DNA损伤和表皮损伤。结论275 nm UVC光具有一定的皮肤安全性,有一定的临床应用前景。

Objective To explore the effects of 275 nm ultraviolet C(UVC)light irradiation on the survival and growth of skin cells and vascular endothelial cells,as well as the effects of long-term and short-term irradiation on the skin of nude mice,and to identify its safety.Methods Human immortalized keratinocytes cells and human umbilical vein endothelial cells were irradiated with 275 nm UVC-LED light source developed,designed and processed by the laboratory for different durations to observe the effects of different irradiation dosages on the survival and growth of the cells;the nude mice skin was irradiated for different durations to observe changes in the appearance of the skin,and tissues of irradiated areas were collected for immunohistochemical analysis.Results For low doses of direct irradiation of cells,275 nm UVC light had certain skin-related cell safety.However,the high dose of 275 nm UVC light irradiation caused more damage to the cells.Meanwhile,neither low-dose nor high-dose irradiation of 275 nm UVC light induced DNA and epidermal damage in nude mice skin.Conclusion The 275 nm UVC light has been identified as promising for clinical applications,and is relatively safe for the skin.